CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008454
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase Nek4 
Protein Synonyms/Alias
 Never in mitosis A-related kinase 4; NimA-related protein kinase 4; Serine/threonine-protein kinase 2; Serine/threonine-protein kinase NRK2 
Gene Name
 NEK4 
Gene Synonyms/Alias
 STK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14CYLRVVGKGSYGEVTubiquitination[1]
30VKHRRDGKQYVIKKLubiquitination[1]
61AQLLSQLKHPNIVTYubiquitination[1]
133HILHRDLKTQNVFLTubiquitination[1]
146LTRTNIIKVGDLGIAubiquitination[1]
209LKHAFNAKDMNSLVYubiquitination[1]
222VYRIIEGKLPPMPRDubiquitination[1]
273SFFLEATKIKTSKNNubiquitination[1]
289KNGDSQSKPFATVVSubiquitination[1]
339PRASGLLKSPASLKAubiquitination[1]
609SSEMSSSKDRPLSARubiquitination[1]
721QTLKLDSKESCEDVPubiquitination[1]
762EAEEIHFKELPSAIMubiquitination[1]
828KYTTYSVKARQLKFFubiquitination[1]
833SVKARQLKFFEENMNubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Seems to act exclusively upon threonine residues (By similarity). 
Sequence Annotation
 DOMAIN 6 261 Protein kinase.
 NP_BIND 12 20 ATP (By similarity).
 ACT_SITE 131 131 Proton acceptor (By similarity).
 BINDING 35 35 ATP (By similarity).
 MOD_RES 165 165 Phosphothreonine; by autocatalysis (By
 MOD_RES 563 563 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Magnesium; Metal-binding; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 841 AA 
Protein Sequence
MPLAAYCYLR VVGKGSYGEV TLVKHRRDGK QYVIKKLNLR NASSRERRAA EQEAQLLSQL 60
KHPNIVTYKE SWEGGDGLLY IVMGFCEGGD LYRKLKEQKG QLLPENQVVE WFVQIAMALQ 120
YLHEKHILHR DLKTQNVFLT RTNIIKVGDL GIARVLENHC DMASTLIGTP YYMSPELFSN 180
KPYNYKSDVW ALGCCVYEMA TLKHAFNAKD MNSLVYRIIE GKLPPMPRDY SPELAELIRT 240
MLSKRPEERP SVRSILRQPY IKRQISFFLE ATKIKTSKNN IKNGDSQSKP FATVVSGEAE 300
SNHEVIHPQP LSSEGSQTYI MGEGKCLSQE KPRASGLLKS PASLKAHTCK QDLSNTTELA 360
TISSVNIDIL PAKGRDSVSD GFVQENQPRY LDASNELGGI CSISQVEEEM LQDNTKSSAQ 420
PENLIPMWSS DIVTGEKNEP VKPLQPLIKE QKPKDQSLAL SPKLECSGTI LAHSNLRLLG 480
SSDSPASASR VAGITGVCHH AQDQVAGECI IEKQGRIHPD LQPHNSGSEP SLSRQRRQKR 540
REQTEHRGEK RQVRRDLFAF QESPPRFLPS HPIVGKVDVT STQKEAENQR RVVTGSVSSS 600
RSSEMSSSKD RPLSARERRR LKQSQEEMSS SGPSVRKASL SVAGPGKPQE EDQPLPARRL 660
SSDCSVTQER KQIHCLSEDE LSSSTSSTDK SDGDYGEGKG QTNEINALVQ LMTQTLKLDS 720
KESCEDVPVA NPVSEFKLHR KYRDTLILHG KVAEEAEEIH FKELPSAIMP GSEKIRRLVE 780
VLRTDVIRGL GVQLLEQVYD LLEEEDEFDR EVRLREHMGE KYTTYSVKAR QLKFFEENMN 840
F 841 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; TAS:ProtInc.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS