UniProt Accession

 MYPT1_HUMAN; O14974; B4DZ09; F8VWB4; Q2NKL4; Q569H0; Q86WU3; Q8NFR6; Q9BYH0 

Genbank Protein ID

 D87930; AY380574; AF458589; AK302692; AC018476; AC073569; AC074270; BC047898; BC092481; BC111752; AB042196 

Genbank Nucleotide ID

 BAA22378.1; AAQ88438.1; AAM49717.1; BAG63921.1; AAH47898.1; AAH92481.1; AAI11753.1; BAB39107.1 

Protein Name

 Protein phosphatase 1 regulatory subunit 12A 

Protein Synonyms/Alias

 Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit 

Gene Name

 PPP1R12A 

Gene Synonyms/Alias

 MBS; MYPT1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
286	YLEELQKKQNLLHSE	ubiquitination	[1]
442	TWRLGLRKTGSYGAL	methylation	[2]
945	QILAENEKLKAQLHD	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Demethylation of RB regulator MYPT1 by histone demethylase LSD1 promotes cell cycle progression in cancer cells.
 Cho HS, Suzuki T, Dohmae N, Hayami S, Unoki M, Yoshimatsu M, Toyokawa G, Takawa M, Chen T, Kurash JK, Field HI, Ponder BA, Nakamura Y, Hamamoto R.
 Cancer Res. 2011 Feb 1;71(3):655-60. [PMID: 21115810] 

Functional Description

 Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity. 

Sequence Annotation

 REPEAT 39 68 ANK 1.
 REPEAT 72 101 ANK 2.
 REPEAT 105 134 ANK 3.
 REPEAT 138 164 ANK 4.
 REPEAT 198 227 ANK 5.
 REPEAT 231 260 ANK 6.
 REGION 682 864 Interaction with ROCK2.
 MOTIF 35 38 KVKF motif.
 MOD_RES 67 67 (3S)-3-hydroxyasparagine; by HIF1AN;
 MOD_RES 100 100 (3S)-3-hydroxyasparagine; by HIF1AN;
 MOD_RES 226 226 (3S)-3-hydroxyasparagine; by HIF1AN;
 MOD_RES 299 299 Phosphoserine.
 MOD_RES 422 422 Phosphoserine.
 MOD_RES 432 432 Phosphoserine.
 MOD_RES 443 443 Phosphothreonine (By similarity).
 MOD_RES 445 445 Phosphoserine; by NUAK1.
 MOD_RES 472 472 Phosphoserine; by NUAK1.
 MOD_RES 473 473 Phosphoserine; by CDK1.
 MOD_RES 477 477 Phosphoserine.
 MOD_RES 507 507 Phosphoserine.
 MOD_RES 601 601 Phosphoserine.
 MOD_RES 668 668 Phosphoserine (By similarity).
 MOD_RES 692 692 Phosphoserine; by PKA and PKG; in vitro.
 MOD_RES 695 695 Phosphoserine; by PKA and PKG; in vitro.
 MOD_RES 696 696 Phosphothreonine; by ROCK1; ROCK2;
 MOD_RES 852 852 Phosphoserine; by ROCK2.
 MOD_RES 862 862 Phosphoserine.
 MOD_RES 871 871 Phosphoserine.
 MOD_RES 910 910 Phosphoserine; by NUAK1.
 MOD_RES 995 995 Phosphoserine (By similarity).  

Keyword

 3D-structure; Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; Direct protein sequencing; Hydroxylation; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1030 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0043292; C:contractile fiber; IDA:UniProtKB.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0072357; C:PTW/PP1 phosphatase complex; IDA:UniProtKB.
 GO:0071889; F:14-3-3 protein binding; IDA:UniProtKB.
 GO:0004857; F:enzyme inhibitor activity; IDA:UniProtKB.
 GO:0019208; F:phosphatase regulator activity; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; NAS:ProtInc.
 GO:0051297; P:centrosome organization; IMP:UniProtKB.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007067; P:mitosis; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006470; P:protein dephosphorylation; IMP:UniProtKB.
 GO:0030155; P:regulation of cell adhesion; IDA:UniProtKB.
 GO:0035507; P:regulation of myosin-light-chain-phosphatase activity; IDA:UniProtKB.
 GO:0046822; P:regulation of nucleocytoplasmic transport; IEA:Compara. 

Interpro

 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR017401; Pase-1_reg_su_12A/B/C_euk. 

Pfam

 PF00023; Ank 

SMART

 SM00248; ANK 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 

PRINTS

 PR01415; ANKYRIN.