CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000560
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 26S proteasome non-ATPase regulatory subunit 4 
Protein Synonyms/Alias
 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; Multiubiquitin chain-binding protein 
Gene Name
 Psmd4 
Gene Synonyms/Alias
 Mcb1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
40VNIVCHSKTRSNPENubiquitination[1]
74DTGRILSKLHTVQPKubiquitination[1]
122SPVEDNEKDLVKLAKubiquitination[1]
126DNEKDLVKLAKRLKKubiquitination[1]
135AKRLKKEKVNVDIINubiquitination[1]
367ALASQATKDGKNDKKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Binds and presumably selects ubiquitin-conjugates for destruction. 
Sequence Annotation
 DOMAIN 5 188 VWFA.
 REPEAT 211 230 UIM 1.
 REPEAT 282 301 UIM 2.
 MOD_RES 250 250 Phosphothreonine.
 MOD_RES 253 253 Phosphothreonine.
 MOD_RES 256 256 Phosphoserine.
 MOD_RES 360 360 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Phosphoprotein; Proteasome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 376 AA 
Protein Sequence
MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV 60
LTTLTPDTGR ILSKLHTVQP KGKITFCTGI RVAHLALKHR QGKNHKMRII AFVGSPVEDN 120
EKDLVKLAKR LKKEKVNVDI INFGEEEVNT EKLTAFVNTL NGKDGTGSHL VTVPPGPSLA 180
DALISSPILA GEGGAMLGLG ASDFEFGVDP SADPELALAL RVSMEEQRQR QEEEARRAAA 240
ASAAEAGIAT PGTEGERDSD DALLKMTINQ QEFGRPGLPD LSSMTEEEQI AYAMQMSLQG 300
TEFSQESADM DASSAMDTSD PVKEEDDYDV MQDPEFLQSV LENLPGVDPN NAAIRSVMGA 360
LASQATKDGK NDKKEEEKK 379 
Gene Ontology
 GO:0022624; C:proteasome accessory complex; IDA:UniProtKB.
 GO:0008540; C:proteasome regulatory particle, base subcomplex; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR027040; Proteasome_su_Rpn10.
 IPR003903; Ubiquitin-int_motif.
 IPR002035; VWF_A. 
Pfam
 PF02809; UIM 
SMART
 SM00726; UIM
 SM00327; VWA 
PROSITE
 PS50330; UIM
 PS50234; VWFA 
PRINTS