UniProt Accession

 CPSF3_MOUSE; Q9QXK7; Q8CIM0 

Genbank Protein ID

 AF203969; BC023297 

Genbank Nucleotide ID

 AAF19420.1; AAH23297.1 

Protein Name

 Cleavage and polyadenylation specificity factor subunit 3 

Protein Synonyms/Alias

 Cleavage and polyadenylation specificity factor 73 kDa subunit; CPSF 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73 

Gene Name

 Cpsf3 

Gene Synonyms/Alias

 Cpsf73 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
295	AMNDKIRKQININNP	ubiquitination	[1]
348	FESWCTDKRNGVIIA	ubiquitination	[1]
381	ITTMSGQKLPLKMSV	ubiquitination	[1]
487	RVSGILVKRNFNYHI	ubiquitination	[1]
545	EELEIQEKPALKVFK	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3' endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. 

Sequence Annotation

 ACT_SITE 396 396 Proton donor (Potential).
 METAL 71 71 Zinc 1 (By similarity).
 METAL 73 73 Zinc 1 (By similarity).
 METAL 75 75 Zinc 2 (By similarity).
 METAL 76 76 Zinc 2 (By similarity).
 METAL 158 158 Zinc 1 (By similarity).
 METAL 179 179 Zinc 1 (By similarity).
 METAL 179 179 Zinc 2 (By similarity).
 METAL 418 418 Zinc 2 (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 681 681 Phosphothreonine (By similarity).
 CROSSLNK 462 462 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 465 465 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 545 545 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; Endonuclease; Hydrolase; Isopeptide bond; Metal-binding; mRNA processing; Nuclease; Nucleus; Phosphoprotein; Reference proteome; Ribonucleoprotein; RNA-binding; Ubl conjugation; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 684 AA 

Protein Sequence

Gene Ontology

 GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; ISS:UniProtKB.
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0008409; F:5'-3' exonuclease activity; IDA:UniProtKB.
 GO:0004521; F:endoribonuclease activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IDA:UniProtKB.
 GO:0006398; P:histone mRNA 3'-end processing; IDA:UniProtKB. 

Interpro

 IPR001279; Beta-lactamas-like.
 IPR022712; Beta_Casp.
 IPR021718; CPSF73-100_C.
 IPR011108; RMMBL. 

Pfam

 PF10996; Beta-Casp
 PF11718; CPSF73-100_C
 PF00753; Lactamase_B
 PF07521; RMMBL 

SMART

 SM01027; Beta-Casp
 SM01098; CPSF73-100_C
 SM00849; Lactamase_B 

PROSITE

  

PRINTS