UniProt Accession

 MED28_HUMAN; Q9H204; Q9BZJ5 

Genbank Protein ID

 AF321617; AF358829; AF318059; AF317680; BC011936 

Genbank Nucleotide ID

 AAG38612.1; AAK32724.1; AAL26906.1; AAK11563.1; AAH11936.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 28 

Protein Synonyms/Alias

 Endothelial-derived protein 1; Mediator complex subunit 28; Merlin and Grb2-interacting cytoskeletal protein; Magicin; Tumor angiogenesis marker EG-1 

Gene Name

 MED28 

Gene Synonyms/Alias

 EG1; FKSG20 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
83	GVDQCIQKFLDIARQ	ubiquitination	[1]
176	ANIPAPLKPT*****	acetylation	[2]

Reference

 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. 

Sequence Annotation

  

Keyword

 Actin-binding; Activator; Coiled coil; Complete proteome; Cytoplasm; Membrane; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 178 AA 

Protein Sequence

Gene Ontology

 GO:0030864; C:cortical actin cytoskeleton; IEA:Compara.
 GO:0016592; C:mediator complex; IEA:Compara.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0051151; P:negative regulation of smooth muscle cell differentiation; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR021640; Mediator_Med28. 

Pfam

 PF11594; Med28 

SMART

  

PROSITE

  

PRINTS