CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006142
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 2 subunit gamma 
Protein Synonyms/Alias
 eIF-2-gamma 
Gene Name
 GCD11 
Gene Synonyms/Alias
 TIF213; YER025W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
64DGETEEEKRKREFEEubiquitination[1]
129GVQTVRFKDELERNIubiquitination[1]
149YANAKIYKCQEPTCPubiquitination[1]
264ESALEHQKSILKFIRacetylation[2]
325IRSFDVNKPGAEIEDacetylation[2]
325IRSFDVNKPGAEIEDubiquitination[1]
334GAEIEDLKGGVAGGSubiquitination[1]
362IRPGIVTKDDKGKIQacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. 
Sequence Annotation
 NP_BIND 107 114 GTP (By similarity).
 NP_BIND 193 197 GTP (By similarity).
 NP_BIND 249 252 GTP (By similarity).
 MOD_RES 60 60 Phosphothreonine.
 MOD_RES 258 258 Phosphoserine.  
Keyword
 Complete proteome; GTP-binding; Initiation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 527 AA 
Protein Sequence
MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA FTGLEEDGET 60
EEEKRKREFE EGGGLPEQPL NPDFSKLNPL SAEIINRQAT INIGTIGHVA HGKSTVVRAI 120
SGVQTVRFKD ELERNITIKL GYANAKIYKC QEPTCPEPDC YRSFKSDKEI SPKCQRPGCP 180
GRYKLVRHVS FVDCPGHDIL MSTMLSGAAV MDAALLLIAG NESCPQPQTS EHLAAIEIMK 240
LKHVIILQNK VDLMREESAL EHQKSILKFI RGTIADGAPI VPISAQLKYN IDAVNEFIVK 300
TIPVPPRDFM ISPRLIVIRS FDVNKPGAEI EDLKGGVAGG SILNGVFKLG DEIEIRPGIV 360
TKDDKGKIQC KPIFSNIVSL FAEQNDLKFA VPGGLIGVGT KVDPTLCRAD RLVGQVVGAK 420
GHLPNIYTDI EINYFLLRRL LGVKTDGQKQ AKVRKLEPNE VLMVNIGSTA TGARVVAVKA 480
DMARLQLTSP ACTEINEKIA LSRRIEKHWR LIGWATIKKG TTLEPIA 527 
Gene Ontology
 GO:0005850; C:eukaryotic translation initiation factor 2 complex; IMP:SGD.
 GO:0043614; C:multi-eIF complex; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IMP:SGD.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0045903; P:positive regulation of translational fidelity; IMP:SGD. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR015256; TIF2_gsu_C.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF09173; eIF2_C
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2 
SMART
  
PROSITE
  
PRINTS
 PR00315; ELONGATNFCT.