UniProt Accession

 SPF30_HUMAN; O75940; B2RA27; D3DRB1; Q5T3K6 

Genbank Protein ID

 AF083385; AF107463; AK314013; AL360182; CH471066; CH471066; CH471066; BC011234 

Genbank Nucleotide ID

 AAC64086.1; AAC84148.1; BAG36724.1; CAI15482.1; EAW49561.1; EAW49562.1; EAW49564.1; AAH11234.1 

Protein Name

 Survival of motor neuron-related-splicing factor 30 

Protein Synonyms/Alias

 30 kDa splicing factor SMNrp; SMN-related protein; Survival motor neuron domain-containing protein 1 

Gene Name

 SMNDC1 

Gene Synonyms/Alias

 SMNR; SPF30 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
165	LKKAQRIKELEQERE	ubiquitination	[1]
219	GTCGIADKPMTQYQD	acetylation	[2, 3, 4, 5]
219	GTCGIADKPMTQYQD	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Necessary for spliceosome assembly. Overexpression causes apoptosis. 

Sequence Annotation

 DOMAIN 72 132 Tudor.
 MOTIF 142 160 Nuclear localization signal (Potential).
 MOD_RES 201 201 Phosphoserine.
 MOD_RES 219 219 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Apoptosis; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 238 AA 

Protein Sequence

Gene Ontology

 GO:0015030; C:Cajal body; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; TAS:ProtInc.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0006917; P:induction of apoptosis; TAS:ProtInc.
 GO:0006397; P:mRNA processing; TAS:ProtInc.
 GO:0000375; P:RNA splicing, via transesterification reactions; TAS:UniProtKB. 

Interpro

 IPR010304; Survival_motor_neuron.
 IPR002999; Tudor. 

Pfam

 PF06003; SMN 

SMART

 SM00333; TUDOR 

PROSITE

 PS50304; TUDOR 

PRINTS