UniProt Accession

 EFNB2_HUMAN; P52799; Q5JV56 

Genbank Protein ID

 U16797; L38734; U81262; AL138689; BC069342; BC074856; BC074857; BC105955; BC105956; BC105957 

Genbank Nucleotide ID

 AAA99707.1; AAC41752.1; AAD03786.1; CAI39907.1; AAH69342.1; AAH74856.1; AAH74857.1; AAI05956.1; AAI05957.1; AAI05958.1 

Protein Name

 Ephrin-B2 

Protein Synonyms/Alias

 EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L 

Gene Name

 EFNB2 

Gene Synonyms/Alias

 EPLG5; HTKL; LERK5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
332	SPANIYYKV******	ubiquitination	[1, 2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. 

Sequence Annotation

 DOMAIN 28 164 Ephrin RBD.
 MOTIF 331 333 PDZ-binding (Potential).
 CARBOHYD 36 36 N-linked (GlcNAc...) (Potential).
 CARBOHYD 139 139 N-linked (GlcNAc...) (Potential).
 DISULFID 62 101
 DISULFID 89 153  

Keyword

 3D-structure; Angiogenesis; Complete proteome; Developmental protein; Differentiation; Disulfide bond; Glycoprotein; Host-virus interaction; Membrane; Neurogenesis; Phosphoprotein; Reference proteome; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 333 AA 

Protein Sequence

Gene Ontology

 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0046875; F:ephrin receptor binding; TAS:ProtInc.
 GO:0007155; P:cell adhesion; IDA:UniProtKB.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0002042; P:cell migration involved in sprouting angiogenesis; IDA:UniProtKB.
 GO:0007267; P:cell-cell signaling; TAS:ProtInc.
 GO:0048013; P:ephrin receptor signaling pathway; IDA:UniProtKB.
 GO:0001945; P:lymph vessel development; IEA:Compara.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0009887; P:organ morphogenesis; IEA:Compara.
 GO:2000727; P:positive regulation of cardiac muscle cell differentiation; ISS:BHF-UCL.
 GO:0050920; P:regulation of chemotaxis; IDA:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR008972; Cupredoxin.
 IPR001799; Ephrin.
 IPR019765; Ephrin_CS. 

Pfam

 PF00812; Ephrin 

SMART

  

PROSITE

 PS01299; EPHRIN_RBD_1
 PS51551; EPHRIN_RBD_2 

PRINTS

 PR01347; EPHRIN.