UniProt Accession

 ATF1_HUMAN; P18846; P25168; Q9H4A8 

Genbank Protein ID

 X55544; BC029619; AJ295163 

Genbank Nucleotide ID

 CAA39150.1; AAH29619.1; CAC15058.1 

Protein Name

 Cyclic AMP-dependent transcription factor ATF-1 

Protein Synonyms/Alias

 cAMP-dependent transcription factor ATF-1; Activating transcription factor 1; Protein TREB36 

Gene Name

 ATF1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
208	TLTSQTTKTDDPQLK	ubiquitination	[1]
215	KTDDPQLKREIRLMK	sumoylation	[2]
234	ARECRRKKKEYVKCL	sumoylation	[2]
239	RKKKEYVKCLENRVA	ubiquitination	[1]
260	KTLIEELKTLKDLYS	ubiquitination	[1, 3, 4, 5]
263	IEELKTLKDLYSNKS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element.
 Iwasaki K, Hailemariam K, Tsuji Y.
 J Biol Chem. 2007 Aug 3;282(31):22335-43. [PMID: 17565989]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation. 

Sequence Annotation

 DOMAIN 31 90 KID.
 DOMAIN 213 271 bZIP.
 REGION 215 239 Basic motif (By similarity).
 REGION 241 262 Leucine-zipper (By similarity).
 MOD_RES 63 63 Phosphoserine; by CaMK1, CDK3, RPS6KA4
 MOD_RES 198 198 Phosphoserine; by HIPK2.  

Keyword

 Activator; Chromosomal rearrangement; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 271 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0051403; P:stress-activated MAPK cascade; TAS:Reactome.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome. 

Interpro

 IPR004827; bZIP.
 IPR003102; Coactivator_CBP_pKID.
 IPR001630; Leuzip_CREB. 

Pfam

 PF00170; bZIP_1
 PF02173; pKID 

SMART

 SM00338; BRLZ 

PROSITE

 PS50217; BZIP
 PS00036; BZIP_BASIC
 PS50953; KID 

PRINTS

 PR00041; LEUZIPPRCREB.