CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006518
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-adrenergic receptor kinase 2 
Protein Synonyms/Alias
 Beta-ARK-2; G-protein-coupled receptor kinase 3 
Gene Name
 ADRBK2 
Gene Synonyms/Alias
 BARK2; GRK3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45SIRSVMQKYLAERNEubiquitination[1]
57RNEITFDKIFNQKIGubiquitination[1]
62FDKIFNQKIGFLLFKubiquitination[1, 2]
115IYDAYIMKELLSCSHubiquitination[1]
170QKFMESDKFTRFCQWubiquitination[1, 2]
319FVVYRDLKPANILLDubiquitination[1]
448GGGSQEVKEHSFFKGubiquitination[1]
454VKEHSFFKGVDWQHVubiquitination[1]
465WQHVYLQKYPPPLIPubiquitination[1]
497EEDTKGIKLLDCDQEubiquitination[1]
507DCDQELYKNFPLVISubiquitination[1, 2]
545ARKRAKNKQLGHEEDubiquitination[1]
645PEFVQWKKELNETFKubiquitination[1]
652KELNETFKEAQRLLRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. 
Sequence Annotation
 DOMAIN 54 175 RGS.
 DOMAIN 191 453 Protein kinase.
 DOMAIN 454 521 AGC-kinase C-terminal.
 DOMAIN 558 652 PH.
 NP_BIND 197 205 ATP (By similarity).
 REGION 1 190 N-terminal.
 ACT_SITE 317 317 Proton acceptor (By similarity).
 BINDING 220 220 ATP (By similarity).  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 688 AA 
Protein Sequence
MADLEAVLAD VSYLMAMEKS KATPAARASK RIVLPEPSIR SVMQKYLAER NEITFDKIFN 60
QKIGFLLFKD FCLNEINEAV PQVKFYEEIK EYEKLDNEED RLCRSRQIYD AYIMKELLSC 120
SHPFSKQAVE HVQSHLSKKQ VTSTLFQPYI EEICESLRGD IFQKFMESDK FTRFCQWKNV 180
ELNIHLTMNE FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 240
IMLSLVSTGD CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE 300
IILGLEHMHN RFVVYRDLKP ANILLDEHGH ARISDLGLAC DFSKKKPHAS VGTHGYMAPE 360
VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTVN VELPDTFSPE 420
LKSLLEGLLQ RDVSKRLGCH GGGSQEVKEH SFFKGVDWQH VYLQKYPPPL IPPRGEVNAA 480
DAFDIGSFDE EDTKGIKLLD CDQELYKNFP LVISERWQQE VTETVYEAVN ADTDKIEARK 540
RAKNKQLGHE EDYALGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL 600
LTMEQILSVE ETQIKDKKCI LFRIKGGKQF VLQCESDPEF VQWKKELNET FKEAQRLLRR 660
APKFLNKPRS GTVELPKPSL CHRNSNGL 688 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0047696; F:beta-adrenergic receptor kinase activity; IEA:EC.
 GO:0004703; F:G-protein coupled receptor kinase activity; IEA:Compara.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004672; F:protein kinase activity; TAS:ProtInc.
 GO:0031623; P:receptor internalization; IDA:UniProtKB.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR000239; GPCR_kinase.
 IPR011009; Kinase-like_dom.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR000342; Regulat_G_prot_signal.
 IPR016137; Regulat_G_prot_signal_superfam.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00169; PH
 PF00069; Pkinase
 PF00615; RGS 
SMART
 SM00233; PH
 SM00315; RGS
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS50003; PH_DOMAIN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50132; RGS 
PRINTS
 PR00717; GPCRKINASE.