UniProt Accession

 MAFA_HUMAN; Q8NHW3 

Genbank Protein ID

 AB086960; AY083269; AC105118 

Genbank Nucleotide ID

 BAC20389.1; AAL89527.1 

Protein Name

 Transcription factor MafA 

Protein Synonyms/Alias

 Pancreatic beta-cell-specific transcriptional activator; Transcription factor RIPE3b1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A 

Gene Name

 MAFA 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
32	DLMKFEVKKEPPEAE	sumoylation	[1, 2]

Reference

 [1] Sumoylation regulates the transcriptional activity of MafA in pancreatic beta cells.
 Shao C, Cobb MH.
 J Biol Chem. 2009 Jan 30;284(5):3117-24. [PMID: 19029092]
 [2] SUMOylation negatively regulates transcriptional and oncogenic activities of MafA.
 Kanai K, Reza HM, Kamitani A, Hamazaki Y, Han SI, Yasuda K, Kataoka K.
 Genes Cells. 2010 Sep 1;15(9):971-82. [PMID: 20718938] 

Functional Description

 Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. 

Sequence Annotation

 DOMAIN 254 317 bZIP.
 REGION 254 279 Basic motif.
 REGION 260 274 Interaction with DNA.
 REGION 282 303 Leucine-zipper.
 MOD_RES 14 14 Phosphoserine; by MAPK1.
 MOD_RES 49 49 Phosphoserine; by GSK3.
 MOD_RES 53 53 Phosphothreonine; by GSK3.
 MOD_RES 57 57 Phosphothreonine; by GSK3.
 MOD_RES 61 61 Phosphoserine; by GSK3.
 MOD_RES 65 65 Phosphoserine; by MAPK1.  

Keyword

 3D-structure; Activator; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Transcription; Transcription regulation; Tumor suppressor; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 353 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; NAS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; TAS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:UniProtKB.
 GO:0030073; P:insulin secretion; IDA:BHF-UCL.
 GO:0007263; P:nitric oxide mediated signal transduction; IDA:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004827; bZIP.
 IPR004826; bZIP_Maf.
 IPR008917; Euk_TF_DNA-bd.
 IPR013592; Maf_TF_N.
 IPR024874; Transciption_factor_Maf. 

Pfam

 PF03131; bZIP_Maf
 PF08383; Maf_N 

SMART

 SM00338; BRLZ 

PROSITE

 PS50217; BZIP
 PS00036; BZIP_BASIC 

PRINTS