CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030817
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase HECW1 
Protein Synonyms/Alias
 cDNA FLJ58788, highly similar to Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA 
Gene Name
 HECW1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
188NSAAPIFKSIGADETubiquitination[1, 2]
504AGREEEEKEQEEEGDubiquitination[1, 2]
592AEEESTLKDSSEKDGubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Ligase; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1572 AA 
Protein Sequence
MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN MDLRGGPHDG 60
VTIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW DIKEEVDAGD WIGMYLIDEV 120
LSENFLDYKN RGVNGSHRGQ IIWKIDASSY FVEPETKICF KYYHGVSGAL RATTPSVTVK 180
NSAAPIFKSI GADETVQGQG SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI 240
FPALPHHGQE RRSKIIGNTV NPIWQAEQFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK 300
LSMPVQRLLE RHAIGDRVVS YTLGRRLPTD HVSGQLQFRF EITSSIHPDD EEISLSTEPE 360
SAQIQDSPMN NLMESGSGEP RSEAPESSES WKPEQLGEGS VPDGPGNQSI ELSRPAEEAA 420
VITEAGDQGM VSVGPEGAGE LLAQVQKDIQ PAPSAEELAE QLDLGEEASA LLLEDGEAPA 480
STKEEPLEEE ATTQSRAGRE EEEKEQEEEG DVSTLEQGEG RLQLRASVKR KSRPCSLPVS 540
ELETVIASAC GDPETPRTHY IRIHTLLHSM PSAQGGSAAE EEDGAEEEST LKDSSEKDGL 600
SEVDTVAADP SALEEDREEP EGATPGTAHP GHSGGHFPSL ANGAAQDGDT HPSTGSESDS 660
SPRQGGDHSC EGCDASCCSP SCYSSSCYST SCYSSSCYSA SCYSPSCYNG NRFASHTRFS 720
SVDSAKISES TVFSSQDDEE EENSAFESVP DSMQSPELDP ESTNGAGPWQ DELAAPSGHV 780
ERSPEGLESP VAGPSNRRED WEARIDSHGR VFYVDHVNRT TTWQRPTAAA TPDGMRRSGS 840
IQQMEQLNRR YQNIQRTIAT ERSEEDSGSQ SCEQAPAGGG GGGGSDSEAE SSQSSLDLRR 900
EGSLSPVNSQ KITLLLQSPA VKFITNPEFF TVLHANYSAY RVFTSSTCLK HMILKVRRDA 960
RNFERYQHNR DLVNFINMFA DTRLELPRGW EIKTDQQGKS FFVDHNSRAT TFIDPRIPLQ 1020
NGRLPNHLTH RQHLQRLRSY SAGEASEVSR NRGASLLARP GHSLVAAIRS QHQHESLPLA 1080
YNDKIVAFLR QPNIFEMLQE RQPSLARNHT LREKIHYIRT EGNHGLEKLS CDADLVILLS 1140
LFEEEIMSYV PLQAAFHPGY SFSPRCSPCS SPQNSPGLQR ASARAPSPYR RDFEAKLRNF 1200
YRKLEAKGFG QGPGKIKLII RRDHLLEGTF NQVMAYSRKE LQRNKLYVTF VGEEGLDYSG 1260
PSREFFFLLS QELFNPYYGL FEYSANDTYT VQISPMSAFV ENHLEWFRFS GRILGLALIH 1320
QYLLDAFFTR PFYKALLRLP CDLSDLEYLD EEFHQSLQWM KDNNITDILD LTFTVNEEVF 1380
GQVTERELKS GGANTQVTEK NKKEYIERMV KWRVERGVVQ QTEALVRGFY EVVDSRLVSV 1440
FDARELELVI AGTAEIDLND WRNNTEYRGG YHDGHLVIRW FWAAVERFNN EQRLRLLQFV 1500
TGTSSVPYEG FAALRGSNGL RRFCIEKWGK ITSLPRAHTC FNRLDLPPYP SYSMLYEKLL 1560
TAVEETSTFG LE 1572 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:GOC. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR000569; HECT.
 IPR001202; WW_dom. 
Pfam
 PF00168; C2
 PF00632; HECT
 PF00397; WW 
SMART
 SM00239; C2
 SM00119; HECTc
 SM00456; WW 
PROSITE
 PS50004; C2
 PS50237; HECT
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS