UniProt Accession

 ADX_MOUSE; P46656 

Genbank Protein ID

 L29123; D43689; D43690 

Genbank Nucleotide ID

 AAA74303.1; BAA07786.1; BAA07787.1 

Protein Name

 Adrenodoxin, mitochondrial 

Protein Synonyms/Alias

 Adrenal ferredoxin; Ferredoxin-1 

Gene Name

 Fdx1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
70	ARSSSEDKITVHFKN	acetylation	[1, 2, 3, 4]
70	ARSSSEDKITVHFKN	succinylation	[3]
76	DKITVHFKNRDGETL	acetylation	[2, 4]
86	DGETLTTKGKIGDSL	acetylation	[2]
162	GCQVCLTKAMDNMTV	acetylation	[1, 2, 3, 4]
162	GCQVCLTKAMDNMTV	succinylation	[3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Essential for the synthesis of various steroid hormones, participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (By similarity). 

Sequence Annotation

 DOMAIN 69 175 2Fe-2S ferredoxin-type.
 METAL 110 110 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 116 116 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 119 119 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 156 156 Iron-sulfur (2Fe-2S) (By similarity).  

Keyword

 2Fe-2S; Cholesterol metabolism; Complete proteome; Electron transport; Iron; Iron-sulfur; Lipid metabolism; Metal-binding; Mitochondrion; Reference proteome; Steroid metabolism; Steroidogenesis; Sterol metabolism; Transit peptide; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 188 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0051537; F:2 iron, 2 sulfur cluster binding; ISS:UniProtKB.
 GO:0009055; F:electron carrier activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008203; P:cholesterol metabolic process; ISS:UniProtKB.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0042446; P:hormone biosynthetic process; ISS:UniProtKB.
 GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR001041; 2Fe-2S_ferredoxin-type.
 IPR001055; Adrenodoxin.
 IPR018298; Adrenodoxin_Fe-S_BS.
 IPR012675; Beta-grasp_dom. 

Pfam

 PF00111; Fer2 

SMART

  

PROSITE

 PS51085; 2FE2S_FER_2
 PS00814; ADX 

PRINTS

 PR00355; ADRENODOXIN.