UniProt Accession

 NDUB8_HUMAN; O95169; A8K0L4; Q5W144; Q9UG53; Q9UJR4; Q9UQF3 

Genbank Protein ID

 AF044958; AF115968; AF077028; AL080056; CR533490; AK289579; AL133352; CH471066; BC000466; BC019276; Y18944 

Genbank Nucleotide ID

 AAD05422.1; AAP97239.1; AAD27761.1; CAB45691.1; CAG38521.1; BAF82268.1; CAH73563.1; EAW49820.1; AAH00466.1; AAH19276.1; CAB46274.1 

Protein Name

 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial 

Protein Synonyms/Alias

 Complex I-ASHI; CI-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit 

Gene Name

 NDUFB8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
34	RTASHMTKDMFPGPY	ubiquitination	[1, 2, 3, 4]
77	MGYGDYPKLPDRSQH	ubiquitination	[2, 3, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. 

Sequence Annotation

  

Keyword

 Complete proteome; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Respiratory chain; Transit peptide; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 186 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:LIFEdb.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; NAS:UniProtKB.
 GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; NAS:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR008699; NADH_UbQ_OxRdtase_ASHI_su.
 IPR016551; NADH_UbQ_OxRdtase_b-cplx_su8. 

Pfam

 PF05821; NDUF_B8 

SMART

  

PROSITE

  

PRINTS