CPLM-010874

Genbank Nucleotide ID

Transcription factor AP-4

Protein Synonyms/Alias

Activating enhancer-binding protein 4; Class C basic helix-loop-helix protein 41; bHLHc41

Homo sapiens (Human)

Position	Peptide	Type	References
10	YFMVPTQKVPSLQHF	ubiquitination	[1]
114	LQQNTQLKRFIQELS	ubiquitination	[1, 2, 3]
189	HMYPEKLKVIAQQVQ	ubiquitination	[1, 3, 4, 5]
299	QRRAVIVKPVRSCPE	ubiquitination	[6]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'- CAGCTG-3'.

DOMAIN 48 99 bHLH.
REGION 100 120 Leucine-zipper 1.
REGION 151 179 Leucine-zipper 2.
MOD_RES 123 123 Phosphoserine.
MOD_RES 124 124 Phosphoserine.
MOD_RES 139 139 Phosphoserine.
MOD_RES 282 282 Phosphothreonine.

Activator; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0017053; C:transcriptional repressor complex; ISS:UniProtKB.
GO:0070888; F:E-box binding; IDA:UniProtKB.
GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
GO:0071549; P:cellular response to dexamethasone stimulus; IEA:Compara.
GO:0006978; P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; IDA:UniProtKB.
GO:0043922; P:negative regulation by host of viral transcription; IDA:UniProtKB.
GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
GO:0071157; P:negative regulation of cell cycle arrest; IMP:UniProtKB.
GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB.
GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:UniProtKB.
GO:0043392; P:negative regulation of DNA binding; IDA:UniProtKB.
GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
GO:0043923; P:positive regulation by host of viral transcription; IDA:UniProtKB.
GO:0043065; P:positive regulation of apoptotic process; IDA:UniProtKB.
GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:UniProtKB.
GO:0006461; P:protein complex assembly; IDA:UniProtKB.

IPR011598; bHLH_dom.