CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011220
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neurofibromin 
Protein Synonyms/Alias
 Neurofibromatosis-related protein NF-1 
Gene Name
 Nf1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
362NLLFNPSKPFSRGSQubiquitination[1]
480TSLKFKEKPTDLETRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity. 
Sequence Annotation
 DOMAIN 1237 1453 Ras-GAP.
 DOMAIN 1582 1740 CRAL-TRIO.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 866 866 Phosphoserine (By similarity).
 MOD_RES 878 878 Phosphoserine (By similarity).
 MOD_RES 2190 2190 Phosphoserine (By similarity).
 MOD_RES 2516 2516 Phosphothreonine.
 MOD_RES 2517 2517 Phosphoserine (By similarity).
 MOD_RES 2523 2523 Phosphoserine (By similarity).
 MOD_RES 2545 2545 Phosphoserine.
 MOD_RES 2819 2819 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; GTPase activation; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2841 AA 
Protein Sequence
MAAHRPVEWV QAVVSRFDEQ LPIKTGQQNT HTKVSTEHNK ECLINISKYK FSLVISGLTT 60
ILKNVNNMRI FGEAAEKNLY LSQLIILDTL EKCLAGQPKD TMRLDETMLV KQLLPEICHF 120
LHTCREGNQH AAELRNSASG VLFSLSCNNF NAVFSRISTR LQELTVCSED NVDVHDIELL 180
QYINVDCAKL KRLLKETAFK FKALKKVAQL AVINSLEKAF WNWVENYPDE FTKLYQIPQT 240
DMAECAEKLF DLVDGFAEST KRKAAVWPLQ IILLILCPEI IQDISKDVVD ESNINKKLFL 300
DSLRKALAGH GGSRQLTESA AIACVKLCKA STYINWEDNS VIFLLVQSMV VDLKNLLFNP 360
SKPFSRGSQP ADVDLMIDCL VSCFRISPHN NQHFKICLAQ NSPSTFHYVL VNSLHRIITN 420
SALDWWPKID AVYCHSVELR NMFGETLHKA VQGCGAHPAI RMAPSLTFKE KVTSLKFKEK 480
PTDLETRSYK CLLLSMVKLI HADPKLLLCN PRKQGPETQS STAELITGLV QLVPQSHMPE 540
VAQEAMEALL VLHQLDSIDL WNPDAPVETF WEISSQMLFY ICKKLTSHQM LSSTEILKWL 600
REILICRNKF LLKNKQADRS SCHSLYLYGV GCEMSATGNT TQMSVDHDEF LRACTPGASL 660
RKGRGNSSMD STAGCSGTPP ICRQAQTKLE VALYMFLWNP DTEAVLVAMS CFRHLCEEAD 720
IRCGVDEVSV HNFLPNYNTF MEFASVSNMM STGRAALQKR VMALLRRIEH PTAGNIEAWE 780
DTHAKWEQAT KLILNYPKAK MEDGQAAESL HKTIVKRRMS HVSGGGSIDL SDTDSLQEWI 840
NMTGFLCALG GVCLQQRSSS GLATYSPPMG AVSERKGSMI SVMSSEGNID SPVSRFMDRL 900
LSLMVCNHEK VGLQIRTNVK DLVGLELSPA LYPMLFNKLK NTISKFFDSQ GQVLLSDSNT 960
QFVEQTIAIM KNLLDNHTEG SSEHLGQASI ETMMLNLVRY VRVLGNMVHA IQIKTKLCQL 1020
VEVMMARRDD LSFCQEMKFR NKMVEYLTDW VMGTSNQAAD DDIKCLTRDL DQASMEAVVS 1080
LLAGLPLQPE EGDGVELMEA KSQLFLKYFT LFMNLLNDCS EVEDENAQTG GRKRGMSRRL 1140
ASLRHCTVLA MSNLLNANVD SGLMHSIGLG YHKDLQTRAT FMEVLTKILQ QGTEFDTLAE 1200
TVLADRFERL VELVTMMGDQ GELPIAMALA NVVPCSQWDE LARVLVTLFD SRHLLYQLLW 1260
NMFSKEVELA DSMQTLFRGN SLASKIMTFC FKVYGATYLQ KLLDPLLRVI ITSSDWQHVS 1320
FEVDPTRLEP SESLEENQRN LLQMTEKFFH AIISSSSEFP SQLRSVCHCL YQATCHSLLN 1380
KATVKERKEN KKSVVSQRFP QNSIGAVGSA MFLRFINPAI VSPYEAGILD KKPPPRIERG 1440
LKLMSKVLQS IANHVLFTKE EHMRPFNDFV KSNFDLARRF FLDIASDCPT SDAVNHSLSF 1500
ISDGNVLALH RLLWNNQEKI GQYLSSNRDH KAVGRRPFDK MATLLAYLGP PEHKPVADTH 1560
WSSLNLTSSK FEEFMTRHQV HEKEEFKALK TLSIFYQAGT SKAGNPIFYY VARRFKTGQI 1620
NGDLLIYHVL LTLKPYYAKP YEIVVDLTHT GPSNRFKTDF LSKWFVVFPG FAYDNVSAVY 1680
IYNCNSWVRE YTKYHERLLT GLKGSKRLIF IDCPGKLAEH IEHEQQKLPA ATLALEEDLK 1740
VFHNALKLAH KDTKVSIKVG STAVQVTSAE RTKVLGQSVF LNDIYYASEI EEICLVDENQ 1800
FTLTIANQGT PLTFMHQECE AIVQSIIHIR TRWELSQPDS IPQHTKIRPK DVPGTLLNIA 1860
LLNLGSSDPS LRSAAYNLLC ALTCTFNLKI EGQLLETSGL CIPANNTLFI VSISKTLAAN 1920
EPHLTLEFLE ECISGFSKSS IELKHLCLEY MTPWLSNLVR FCKHNDDAKR QRVTAILDKL 1980
ITMTINEKQM YPSIQAKIWG SLGQITDLLD VVLDSFIKTS ATGGLGSIKA EVMADTAVAL 2040
ASGNVKLVSS KVIGRMCKII DKTCLSPTPT LEQHLMWDDI AILARYMLML SFNNSLDVAA 2100
HLPYLFHVVT FLVATGPLSL RASTHGLLIN IIHSLCTCSQ LHFSEETKQV LRLSLTEFSL 2160
PKFYLLFGIS KVKSAAVIAF RSSYRDRSFS PGSYERETFA LTSLETVTEA LLEIMEACMR 2220
DIPTCKWLDQ WTELAQRFAF QYNPSLQPRA LVVFGCISKR VSHGQIKQII RILSKALESC 2280
LKGPDTYNSQ VLIESTVIAL TKLQPLLNKD SPLHKALFWV AVAVLQLDEV NLYSAGTALL 2340
EQNLHTLDSL RIFNDKSPEE VFMAIRNPLE WHCKQMDHFV GLNFNSNFNF ALVGHLLKGY 2400
RHPSPAIVAR TVRILHTLLT LVNKHRNCDK FEVNTQSVAY LAALLTVSEE VRSRCSLKHR 2460
KSLLLTDISM ENVPMDTYPI HHGDPSYRTL KETQPWSSPK GSEGYLAATY PAVGQTSPRA 2520
RKSMSLDMGQ PSQANTKKLL GTRKSFDHLI SDTKAPKRQE MESGITTPPK MRRVAETDYE 2580
METQRIPSSQ QHPHLRKVSV SESNVLLDEE VLTDPKIQAL LLTVLATLVK YTTDEFDQRI 2640
LYEYLAEASV VFPKVFPVVH NLLDSKINTL LSLCQDPNLL NPIHGIVQSV VYHEESPPQY 2700
QTSYLQSFGF NGLWRFAGPF SKQTQIPDYA ELIVKFLDAL IDTYLPGIDE ETSEESLLTP 2760
TSPYPPALQS QLSITANLNL SNSMTSLATS QHSPGLDKEN VELSPTAGHC NSGRTRHGSA 2820
SQVQKQRSAG SFKRNSIKKI V 2841 
Gene Ontology
 GO:0030424; C:axon; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0031235; C:intrinsic to internal side of plasma membrane; IBA:RefGenome.
 GO:0005099; F:Ras GTPase activator activity; IGI:MGI.
 GO:0030036; P:actin cytoskeleton organization; IMP:MGI.
 GO:0030325; P:adrenal gland development; IMP:MGI.
 GO:0048844; P:artery morphogenesis; IMP:MGI.
 GO:0048593; P:camera-type eye morphogenesis; IMP:MGI.
 GO:0021987; P:cerebral cortex development; IMP:MGI.
 GO:0030199; P:collagen fibril organization; IMP:MGI.
 GO:0021897; P:forebrain astrocyte development; IMP:MGI.
 GO:0048853; P:forebrain morphogenesis; IMP:MGI.
 GO:0007507; P:heart development; IMP:MGI.
 GO:0001889; P:liver development; IMP:MGI.
 GO:0000165; P:MAPK cascade; IMP:MGI.
 GO:0001656; P:metanephros development; IMP:MGI.
 GO:0022011; P:myelination in peripheral nervous system; IMP:MGI.
 GO:0016525; P:negative regulation of angiogenesis; IGI:MGI.
 GO:0048712; P:negative regulation of astrocyte differentiation; IMP:MGI.
 GO:0030336; P:negative regulation of cell migration; IMP:MGI.
 GO:0001953; P:negative regulation of cell-matrix adhesion; IGI:MGI.
 GO:0001937; P:negative regulation of endothelial cell proliferation; IMP:MGI.
 GO:0048147; P:negative regulation of fibroblast proliferation; IMP:BHF-UCL.
 GO:0043407; P:negative regulation of MAP kinase activity; IMP:MGI.
 GO:0007406; P:negative regulation of neuroblast proliferation; IMP:MGI.
 GO:0046929; P:negative regulation of neurotransmitter secretion; IMP:MGI.
 GO:0048715; P:negative regulation of oligodendrocyte differentiation; IMP:MGI.
 GO:0045671; P:negative regulation of osteoclast differentiation; IGI:MGI.
 GO:0035021; P:negative regulation of Rac protein signal transduction; IGI:MGI.
 GO:0042992; P:negative regulation of transcription factor import into nucleus; IMP:MGI.
 GO:0021915; P:neural tube development; IGI:MGI.
 GO:0001649; P:osteoblast differentiation; IMP:MGI.
 GO:0014065; P:phosphatidylinositol 3-kinase cascade; IMP:MGI.
 GO:0043473; P:pigmentation; IMP:MGI.
 GO:0045762; P:positive regulation of adenylate cyclase activity; IMP:MGI.
 GO:0001938; P:positive regulation of endothelial cell proliferation; IGI:MGI.
 GO:0043525; P:positive regulation of neuron apoptotic process; IMP:MGI.
 GO:0007265; P:Ras protein signal transduction; IMP:MGI.
 GO:0043535; P:regulation of blood vessel endothelial cell migration; IEA:Compara.
 GO:0045124; P:regulation of bone resorption; IMP:MGI.
 GO:0048169; P:regulation of long-term neuronal synaptic plasticity; IMP:MGI.
 GO:0032228; P:regulation of synaptic transmission, GABAergic; IGI:MGI.
 GO:0001666; P:response to hypoxia; IMP:MGI.
 GO:0007519; P:skeletal muscle tissue development; IMP:MGI.
 GO:0048745; P:smooth muscle tissue development; IMP:MGI.
 GO:0021510; P:spinal cord development; IMP:MGI.
 GO:0048485; P:sympathetic nervous system development; IMP:MGI.
 GO:0008542; P:visual learning; IMP:MGI.
 GO:0042060; P:wound healing; IMP:MGI. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR001251; CRAL-TRIO_dom.
 IPR001936; RasGAP.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot. 
Pfam
 PF00616; RasGAP 
SMART
 SM00323; RasGAP
 SM00516; SEC14 
PROSITE
 PS50191; CRAL_TRIO
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2 
PRINTS