CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000995
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sorting nexin-2 
Protein Synonyms/Alias
 Transformation-related gene 9 protein; TRG-9 
Gene Name
 SNX2 
Gene Synonyms/Alias
 TRG9 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
116IAPRIESKSMSAPVIubiquitination[1, 2, 3, 4]
197LHSKLASKYLHVGYIubiquitination[3]
211IVPPAPEKSIVGMTKubiquitination[3]
218KSIVGMTKVKVGKEDubiquitination[3]
234SSTEFVEKRRAALERubiquitination[3]
248RYLQRTVKHPTLLQDubiquitination[1, 3, 4]
287GILRMVNKAADAVNKubiquitination[3]
447PDKIQQAKNEIREWEacetylation[5]
469RDFEQISKTIRKEVGacetylation[5]
516EAFLPEAKAIA****ubiquitination[3, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572
Functional Description
 May be involved in several stages of intracellular trafficking. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). 
Sequence Annotation
 DOMAIN 140 269 PX.
 BINDING 183 183 Phosphatidylinositol 3-phosphate (By
 BINDING 185 185 Phosphatidylinositol 3-phosphate; via
 BINDING 211 211 Phosphatidylinositol 3-phosphate (By
 BINDING 235 235 Phosphatidylinositol 3-phosphate (By
 MOD_RES 119 119 Phosphoserine.
 MOD_RES 185 185 Phosphoserine.
 MOD_RES 277 277 Phosphoserine.
 MOD_RES 469 469 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Endosome; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 519 AA 
Protein Sequence
MAAEREPPPL GDGKPTDFED LEDGEDLFTS TVSTLESSPS SPEPASLPAE DISANSNGPK 60
PTEVVLDDDR EDLFAEATEE VSLDSPEREP ILSSEPSPAV TPVTPTTLIA PRIESKSMSA 120
PVIFDRSREE IEEEANGDIF DIEIGVSDPE KVGDGMNAYM AYRVTTKTSL SMFSKSEFSV 180
KRRFSDFLGL HSKLASKYLH VGYIVPPAPE KSIVGMTKVK VGKEDSSSTE FVEKRRAALE 240
RYLQRTVKHP TLLQDPDLRQ FLESSELPRA VNTQALSGAG ILRMVNKAAD AVNKMTIKMN 300
ESDAWFEEKQ QQFENLDQQL RKLHVSVEAL VCHRKELSAN TAAFAKSAAM LGNSEDHTAL 360
SRALSQLAEV EEKIDQLHQE QAFADFYMFS ELLSDYIRLI AAVKGVFDHR MKCWQKWEDA 420
QITLLKKREA EAKMMVANKP DKIQQAKNEI REWEAKVQQG ERDFEQISKT IRKEVGRFEK 480
ERVKDFKTVI IKYLESLVQT QQQLIKYWEA FLPEAKAIA 519 
Gene Ontology
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0010008; C:endosome membrane; IDA:UniProtKB.
 GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0006897; P:endocytosis; TAS:ProtInc.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 
Interpro
 IPR001683; Phox.
 IPR005329; Sorting_nexin_N.
 IPR015404; Vps5_C. 
Pfam
 PF00787; PX
 PF03700; Sorting_nexin
 PF09325; Vps5 
SMART
 SM00312; PX 
PROSITE
 PS50195; PX 
PRINTS