CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023723
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Krueppel-like factor 13 
Protein Synonyms/Alias
 Basic transcription element-binding protein 3; BTE-binding protein 3; Novel Sp1-like zinc finger transcription factor 1; RANTES factor of late activated T-lymphocytes 1; RFLAT-1; Transcription factor BTEB3; Transcription factor NSLP1 
Gene Name
 KLF13 
Gene Synonyms/Alias
 BTEB3; NSLP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56VEERRDGKDSASLFVubiquitination[1, 2]
166DLESPQRKHKCHYAGacetylation[3]
168ESPQRKHKCHYAGCEacetylation[3]
180GCEKVYGKSSHLKAHacetylation[3]
206CSWQDCNKKFARSDEubiquitination[4]
207SWQDCNKKFARSDELubiquitination[1]
225YRTHTGEKKFSCPICacetylation[3]
226RTHTGEKKFSCPICEacetylation[3]
226RTHTGEKKFSCPICEubiquitination[1]
234FSCPICEKRFMRSDHacetylation[3]
234FSCPICEKRFMRSDHubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Functional interplay between CBP and PCAF in acetylation and regulation of transcription factor KLF13 activity.
 Song CZ, Keller K, Chen Y, Stamatoyannopoulos G.
 J Mol Biol. 2003 May 30;329(2):207-15. [PMID: 12758070]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Represses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES expression in T-cells. 
Sequence Annotation
 ZN_FING 167 191 C2H2-type 1.
 ZN_FING 197 221 C2H2-type 2.
 ZN_FING 227 249 C2H2-type 3.  
Keyword
 Activator; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 288 AA 
Protein Sequence
MAAAAYVDHF AAECLVSMSS RAVVHGPREG PESRPEGAAV AATPTLPRVE ERRDGKDSAS 60
LFVVARILAD LNQQAPAPAP AERREGAAAR KARTPCRLPP PAPEPTSPGA EGAAAAPPSP 120
AWSEPEPEAG LEPEREPGPA GSGEPGLRQR VRRGRSRADL ESPQRKHKCH YAGCEKVYGK 180
SSHLKAHLRT HTGERPFACS WQDCNKKFAR SDELARHYRT HTGEKKFSCP ICEKRFMRSD 240
HLTKHARRHA NFHPGMLQRR GGGSRTGSLS DYSRSDASSP TISPASSP 288 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0045647; P:negative regulation of erythrocyte differentiation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS