CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044129
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Hepatocyte growth factor-regulated tyrosine kinase substrate 
Protein Synonyms/Alias
  
Gene Name
 HGS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19VHDEVANKQTMEELKubiquitination[1, 2, 3, 4, 5]
26KQTMEELKDLLKRQVubiquitination[1]
30EELKDLLKRQVEVNVubiquitination[6]
59FRNEPKYKVVQDTYQubiquitination[1, 2, 3, 4, 5, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 71 AA 
Protein Sequence
MESVVKNCGQ TVHDEVANKQ TMEELKDLLK RQVEVNVRNK ILYLIQAWAH AFRNEPKYKV 60
VQDTYQIMKV E 71 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0030141; C:secretory granule; IEA:Compara.
 GO:0008333; P:endosome to lysosome transport; IEA:Compara.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0009966; P:regulation of signal transduction; IEA:InterPro. 
Interpro
 IPR008942; ENTH_VHS.
 IPR027426; HGS.
 IPR002014; VHS. 
Pfam
 PF00790; VHS 
SMART
  
PROSITE
 PS50179; VHS 
PRINTS