CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021222
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bromodomain-containing protein 8 
Protein Synonyms/Alias
 Skeletal muscle abundant protein; Skeletal muscle abundant protein 2; Thyroid hormone receptor coactivating protein of 120 kDa; TrCP120; p120 
Gene Name
 BRD8 
Gene Synonyms/Alias
 SMAP; SMAP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
85PKRKRGEKGEVVETVacetylation[1]
481PAPEMTVKQERLDFEacetylation[2]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. 
Sequence Annotation
 DOMAIN 724 794 Bromo 1.
 DOMAIN 1120 1190 Bromo 2.
 MOD_RES 387 387 Phosphoserine.
 MOD_RES 481 481 N6-acetyllysine.
 MOD_RES 621 621 Phosphoserine.
 MOD_RES 637 637 Phosphoserine.
 MOD_RES 641 641 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Bromodomain; Chromatin regulator; Coiled coil; Complete proteome; Direct protein sequencing; Growth regulation; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1235 AA 
Protein Sequence
MATGTGKHKL LSTGPTEPWS IREKLCLASS VMRSGDQNWV SVSRAIKPFA EPGRPPDWFS 60
QKHCASQYSE LLETTETPKR KRGEKGEVVE TVEDVIVRKL TAERVEELKK VIKETQERYR 120
RLKRDAELIQ AGHMDSRLDE LCNDIATKKK LEEEEAEVKR KATDAAYQAR QAVKTPPRRL 180
PTVMVRSPID SASPGGDYPL GDLTPTTMEE ATSGVNESEM AVASGHLNST GVLLEVGGVL 240
PMIHGGEIQQ TPNTVAASPA ASGAPTLSRL LEAGPTQFTT PLASFTTVAS EPPVKLVPPP 300
VESVSQATIV MMPALPAPSS APAVSTTESV APVSQPDNCV PMEAVGDPHT VTVSMDSSEI 360
SMIINSIKEE CFRSGVAEAP VGSKAPSIDG KEELDLAEKM DIAVSYTGEE LDFETVGDII 420
AIIEDKVDDH PEVLDVAAVE AALSFCEEND DPQSLPGPWE HPIQQERDKP VPLPAPEMTV 480
KQERLDFEET ENKGIHELVD IREPSAEIKV EPAEPEPVIS GAEIVAGVVP ATSMEPPELR 540
SQDLDEELGS TAAGEIVEAD VAIGKGDETP LTNVKTEASP ESMLSPSHGS NPIEDPLEAE 600
TQHKFEMSDS LKEESGTIFG SQIKDAPGED EEEDGVSEAA SLEEPKEEDQ GEGYLSEMDN 660
EPPVSESDDG FSIHNATLQS HTLADSIPSS PASSQFSVCS EDQEAIQAQK IWKKAIMLVW 720
RAAANHRYAN VFLQPVTDDI APGYHSIVQR PMDLSTIKKN IENGLIRSTA EFQRDIMLMF 780
QNAVMYNSSD HDVYHMAVEM QRDVLEQIQQ FLATQLIMQT SESGISAKSL RGRDSTRKQD 840
ASEKDSVPMG SPAFLLSLFM GHEWVWLDSE QDHPNDSELS NDCRSLFSSW DSSLDLDVGN 900
WRETEDPEAE ELEESSPERE PSELLVGDGG SEESQEAARK ASHQNLLHFL SEVAYLMEPL 960
CISSNESSEG CCPPSGTRQE GREIKASEGE RELCRETEEL SAKGDPLVAE KPLGENGKPE 1020
VASAPSVICT VQGLLTESEE GEAQQESKGE DQGEVYVSEM EDQPPSGECD DAFNIKETPL 1080
VDTLFSHATS SKLTDLSQDD PVQDHLLFKK TLLPVWKMIA SHRFSSPFLK PVSERQAPGY 1140
KDVVKRPMDL TSLKRNLSKG RIRTMAQFLR DLMLMFQNAV MYNDSDHHVY HMAVEMRQEV 1200
LEQIQVLNIW LDKRKGSSSL EGEPANPVDD GKPVF 1235 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0035267; C:NuA4 histone acetyltransferase complex; IDA:UniProtKB.
 GO:0004887; F:thyroid hormone receptor activity; TAS:ProtInc.
 GO:0007166; P:cell surface receptor signaling pathway; TAS:ProtInc.
 GO:0043968; P:histone H2A acetylation; IDA:UniProtKB.
 GO:0043967; P:histone H4 acetylation; IDA:UniProtKB.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW. 
Interpro
 IPR001487; Bromodomain. 
Pfam
 PF00439; Bromodomain 
SMART
 SM00297; BROMO 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2 
PRINTS
 PR00503; BROMODOMAIN.