CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021557
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein transport protein Sec61 subunit alpha isoform 2 
Protein Synonyms/Alias
 Sec61 alpha-2 
Gene Name
 SEC61A2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MGIKFLEVIKPubiquitination[1]
21AVLPEIQKPERKIQFubiquitination[2]
107IEVGDTPKDRALFNGubiquitination[2, 3]
268FRVDLPIKSARYRGQubiquitination[3]
388EVSGSSAKDVAKQLKubiquitination[1, 3]
392SSAKDVAKQLKEQQMubiquitination[1, 3]
395KDVAKQLKEQQMVMRubiquitination[1, 2, 3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins. Found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins (By similarity). 
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Endoplasmic reticulum; Membrane; Protein transport; Reference proteome; Translocation; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 476 AA 
Protein Sequence
MGIKFLEVIK PFCAVLPEIQ KPERKIQFRE KVLWTAITLF IFLVCCQIPL FGIMSSDSAD 60
PFYWMRVILA SNRGTLMELG ISPIVTSGLI MQLLAGAKII EVGDTPKDRA LFNGAQKLFG 120
MIITIGQAIV YVMTGMYGDP AEMGAGICLL IIIQLFVAGL IVLLLDELLQ KGYGLGSGIS 180
LFIATNICET IVWKAFSPTT INTGRGTEFE GAVIALFHLL ATRTDKVRAL REAFYRQNLP 240
NLMNLIATVF VFAVVIYFQG FRVDLPIKSA RYRGQYSSYP IKLFYTSNIP IILQSALVSN 300
LYVISQMLSV RFSGNFLVNL LGQWADVSGG GPARSYPVGG LCYYLSPPES MGAIFEDPVH 360
VVVYIIFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV MRGHRDTSMV HELNRYIPTA 420
AAFGGLCIGA LSVLADFLGA IGSGTGILLA VTIIYQYFEI FVKEQAEVGG MGALFF 476 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043022; F:ribosome binding; IEA:Compara.
 GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR002208; SecY/SEC61-alpha.
 IPR023201; SecY_su_dom.
 IPR019561; Translocon_Sec61/SecY_plug_dom. 
Pfam
 PF10559; Plug_translocon
 PF00344; SecY 
SMART
  
PROSITE
 PS00755; SECY_1
 PS00756; SECY_2 
PRINTS