UniProt Accession

 CUE5_YEAST; Q08412; D6W2A8 

Genbank Protein ID

 Z74949; AY558044; BK006948 

Genbank Nucleotide ID

 CAA99232.1; AAS56370.1; DAA10824.1 

Protein Name

 Ubiquitin-binding protein CUE5 

Protein Synonyms/Alias

 Coupling of ubiquitin conjugation to ER degradation protein 5 

Gene Name

 CUE5 

Gene Synonyms/Alias

 YOR042W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
4	****MEEKEGIKDSS	ubiquitination	[1]
8	MEEKEGIKDSSLLEK	ubiquitination	[2]
15	KDSSLLEKSNVPESI	ubiquitination	[1, 2]
28	SINEDISKTTDVDLN	ubiquitination	[2]
39	VDLNSDGKKDNDTSA	ubiquitination	[1, 2]
47	KDNDTSAKDGTPKVE	ubiquitination	[2]
56	GTPKVEEKVNKSSGI	ubiquitination	[2]
59	KVEEKVNKSSGIDED	ubiquitination	[1]
76	VTPAEDAKEEEEEHP	ubiquitination	[1, 2, 3, 4, 5]
97	KSEEEPSKENPILQE	ubiquitination	[2]
156	LPTQPVRKNPEAPAR	ubiquitination	[1, 2]
232	SWSQFVEKDLPELTD	ubiquitination	[2]
251	SLQDTANKVSNWISD	ubiquitination	[2]
354	KSGESTGKVVAETTY	ubiquitination	[1, 2]
370	DTPDTETKKKWQPLP	ubiquitination	[1, 2]
371	TPDTETKKKWQPLPP	ubiquitination	[1]
372	PDTETKKKWQPLPPE	ubiquitination	[2]
387	PLDTTPTKVNAVSRN	ubiquitination	[2]
395	VNAVSRNKKNPDEDE	ubiquitination	[2]
396	NAVSRNKKNPDEDEF	ubiquitination	[2, 3, 4, 5]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [4] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [5] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Binds ubiquitin via its CUE domain which may facilitate intramolecular monoubiquitination. 

Sequence Annotation

 DOMAIN 97 140 CUE.
 MOD_RES 70 70 Phosphothreonine.
 MOD_RES 220 220 Phosphoserine.
 MOD_RES 384 384 Phosphothreonine.
 MOD_RES 407 407 Phosphoserine.
 CROSSLNK 76 76 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 396 396 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Complete proteome; Cytoplasm; Isopeptide bond; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 411 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD. 

Interpro

 IPR003892; CUE.
 IPR009060; UBA-like. 

Pfam

 PF02845; CUE 

SMART

 SM00546; CUE 

PROSITE

 PS51140; CUE 

PRINTS