CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008958
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nck-associated protein 1 
Protein Synonyms/Alias
 NAP 1; Membrane-associated protein HEM-2; p125Nap1 
Gene Name
 Nckap1 
Gene Synonyms/Alias
 Hem2; Nap1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
12VLQPSQQKLAEKLTIubiquitination[1]
16SQQKLAEKLTILNDRubiquitination[1]
35LTRLYNIKKACGDPKacetylation[2]
886QMRTSFDKPDQMAALubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1 (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine (By similarity).  
Keyword
 Acetylation; Cell membrane; Cell projection; Complete proteome; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1128 AA 
Protein Sequence
MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID KNLESAVKFI 60
VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV MEFKDHVCDL LNTIDVCQVF 120
FDITVNFDLT KNYLDLTVTY TTLMILLSRI EERKAIIGLY NYAHEMTHGA SDREYPRLGQ 180
MIVDYEHPLK KMMEEFVPHS KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML 240
NPAQSDTMPC EYLSLDAMEK WIIFGFILCH GMLNTEATAL NLWKLALQSS SCLSLFRDEV 300
FHIHKAAEDL FVNIRGYNKR INDIRECKEA AVSHAGSMHR ERRKFLRSAL KELATVLSDQ 360
PGLLGPKALF VFMALSFARD EIIWLLRHAD NMPKKSADDF IDKHIAELIF YMEELRAHVR 420
KYGPVMQRYY VQYLSGFDAV VLNELVQNLS VCPEDESIIM SSFVNTMTSL SVKQVEDGEV 480
FDFRGMRLDW FRLQAYTSVS KASLSLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS 540
IFCFYSRAFE KMFQQCLELP SQSRYSIAFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN 600
MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG KKGEPEREKP 660
GVESMRKNRL VVTNLDKLHT ALSELCFSIN YVPNMAVWEH TFTPREYLTS HLEIRFTKSI 720
VGMTMYNQAT QEIAKPSELL TSVREYMTVL QSIENYVQID ITRVFNNVLL QQTQHLDSHG 780
EPTITSLYTN WYLETLLRQV SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS 840
ELLGPYGMKF LSESLMWHIS SQVAELKKLV VENVDVLTQM RTSFDKPDQM AALFKRLSSV 900
DSVLKRMTII GVILSFRSLA QEALRDVLSY HIPFLVSSIE DFKDHIPRET DMKVAMNVYE 960
LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM VFVAVSLPTL ASNVMSQYSP 1020
AIEGHCNNIH CLAKAINQIA AALFTIHKGS IEDRLKEFLA LASSSLLKIG QETDKTTTRN 1080
RESVYLLLDM IVQESPFLTM DLLESCFPYV LLRNAYHAVY KQSVTSSA 1128 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell. 
Interpro
 IPR019137; Nck-associated_protein-1. 
Pfam
 PF09735; Nckap1 
SMART
  
PROSITE
  
PRINTS