CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000406
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dihydroxyacetone phosphate acyltransferase 
Protein Synonyms/Alias
 DAP-AT; DHAP-AT; Acyl-CoA:dihydroxyacetonephosphateacyltransferase; Glycerone-phosphate O-acyltransferase 
Gene Name
 GNPAT 
Gene Synonyms/Alias
 DAPAT; DHAPAT 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
113ILDEMSHKLRLGAIRubiquitination[1, 2, 3]
146VNEEGIQKLQRAIQEubiquitination[2]
312ESTTGLLKARKILSEubiquitination[2]
643VRLGVVEKKKINNNCacetylation[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
  
Sequence Annotation
 MOTIF 162 167 HXXXXD motif.
 MOTIF 678 680 Microbody targeting signal (Potential).
 MOD_RES 643 643 N6-acetyllysine.  
Keyword
 Acetylation; Acyltransferase; Cataract; Complete proteome; Direct protein sequencing; Disease mutation; Dwarfism; Membrane; Peroxisome; Reference proteome; Rhizomelic chondrodysplasia punctata; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 680 AA 
Protein Sequence
MESSSSSNSY FSVGPTSPSA VVLLYSKELK KWDEFEDILE ERRHVSDLKF AMKCYTPLVY 60
KGITPCKPID IKCSVLNSEE IHYVIKQLSK ESLQSVDVLR EEVSEILDEM SHKLRLGAIR 120
FCAFTLSKVF KQIFSKVCVN EEGIQKLQRA IQEHPVVLLP SHRSYIDFLM LSFLLYNYDL 180
PVPVIAAGMD FLGMKMVGEL LRMSGAFFMR RTFGGNKLYW AVFSEYVKTM LRNGYAPVEF 240
FLEGTRSRSA KTLTPKFGLL NIVMEPFFKR EVFDTYLVPI SISYDKILEE TLYVYELLGV 300
PKPKESTTGL LKARKILSEN FGSIHVYFGD PVSLRSLAAG RMSRSSYNLV PRYIPQKQSE 360
DMHAFVTEVA YKMELLQIEN MVLSPWTLIV AVLLQNRPSM DFDALVEKTL WLKGLTQAFG 420
GFLIWPDNKP AEEVVPASIL LHSNIASLVK DQVILKVDSG DSEVVDGLML QHITLLMCSA 480
YRNQLLNIFV RPSLVAVALQ MTPGFRKEDV YSCFRFLRDV FADEFIFLPG NTLKDFEEGC 540
YLLCKSEAIQ VTTKDILVTE KGNTVLEFLV GLFKPFVESY QIICKYLLSE EEDHFSEEQY 600
LAAVRKFTSQ LLDQGTSQCY DVLSSDVQKN ALAACVRLGV VEKKKINNNC IFNVNEPATT 660
KLEEMLGCKT PIGKPATAKL 680 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005782; C:peroxisomal matrix; TAS:Reactome.
 GO:0005778; C:peroxisomal membrane; IDA:UniProtKB.
 GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:InterPro.
 GO:0016287; F:glycerone-phosphate O-acyltransferase activity; IDA:UniProtKB.
 GO:0016290; F:palmitoyl-CoA hydrolase activity; IDA:UniProtKB.
 GO:0016044; P:cellular membrane organization; IEA:Compara.
 GO:0021587; P:cerebellum morphogenesis; IEA:Compara.
 GO:0008611; P:ether lipid biosynthetic process; IDA:UniProtKB.
 GO:0030913; P:paranodal junction assembly; IEA:Compara.
 GO:0006654; P:phosphatidic acid biosynthetic process; TAS:Reactome.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0070542; P:response to fatty acid; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0042594; P:response to starvation; IEA:Compara.
 GO:0007416; P:synapse assembly; IEA:Compara. 
Interpro
 IPR022284; G3P_O-AcylTrfase.
 IPR002123; Plipid/glycerol_acylTrfase. 
Pfam
 PF01553; Acyltransferase 
SMART
 SM00563; PlsC 
PROSITE
  
PRINTS