CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030686
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Elongator complex protein 3 
Protein Synonyms/Alias
 cDNA FLJ57255, highly similar to Homo sapiens elongation protein 3 homolog (ELP3), mRNA; cDNA, FLJ78884, highly similar to Homo sapiens elongation protein 3 homolog (ELP3), mRNA 
Gene Name
 ELP3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37RHRIEQLKQLGHSVDubiquitination[1]
87NNIYEAVKYSERSLTubiquitination[1, 2, 3, 4, 5, 6]
95YSERSLTKCIGITIEubiquitination[4]
156CESFHLAKDSGFKVVubiquitination[4, 6]
161LAKDSGFKVVAHMMPacetylation[7]
161LAKDSGFKVVAHMMPubiquitination[4]
214TGLYELWKSGRYKSYubiquitination[1, 2, 4, 5, 6]
219LWKSGRYKSYSPSDLubiquitination[1, 2, 4, 5]
273ELALARMKDLGIQCRubiquitination[4]
295GIQEIHHKVRPYQVEubiquitination[4, 6]
372VSSRDPTKFQHQGFGubiquitination[1]
398REEHGSGKIAVISGVubiquitination[1, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 428 AA 
Protein Sequence
MSTHQFYRKY MCPTSMRAIR ARYDPFLQTR HRIEQLKQLG HSVDKVEFIV MGGTFMALPE 60
EYRDYFIRNL HDALSGHTSN NIYEAVKYSE RSLTKCIGIT IETRPDYCMK RHLSDMLTYG 120
CTRLEIGVQS VYEDVARDTN RGHTVKAVCE SFHLAKDSGF KVVAHMMPDL PNVGLERDIE 180
QFTEFFENPA FRPDGLKLYP TLVIRGTGLY ELWKSGRYKS YSPSDLVELV ARILALVPPW 240
TRVYRVQRDI PMPLVSSGVE HGNLRELALA RMKDLGIQCR DVRTREVGIQ EIHHKVRPYQ 300
VELVRRDYVA NGGWETFLSY EDPDQDILIG LLRLRKCSEE TFRFELGGGV SIVRELHVYG 360
SVVPVSSRDP TKFQHQGFGM LLMEEAERIA REEHGSGKIA VISGVGTRNY YRKIGYRLQG 420
PYMVKMLK 428 
Gene Ontology
 GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
 GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
 GO:0008152; P:metabolic process; IEA:GOC. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR006638; Elp3/MiaB/NifB.
 IPR000182; GNAT_dom.
 IPR005910; Hist_AcTrfase_ELP3.
 IPR007197; rSAM.
 IPR023404; rSAM_horseshoe. 
Pfam
 PF00583; Acetyltransf_1
 PF04055; Radical_SAM 
SMART
 SM00729; Elp3 
PROSITE
 PS51186; GNAT 
PRINTS