CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006031
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 14-3-3 protein beta/alpha 
Protein Synonyms/Alias
 Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; 14-3-3 protein beta/alpha, N-terminally processed 
Gene Name
 YWHAB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
3*****MDKSELVQKAubiquitination[1]
9DKSELVQKAKLAEQAubiquitination[1, 2, 3]
11SELVQKAKLAEQAERubiquitination[1]
68VISSIEQKTERNEKKubiquitination[3]
122KVFYLKMKGDYFRYLubiquitination[3]
138EVASGDNKQTTVSNSubiquitination[3]
157QEAFEISKKEMQPTHubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine; in 14-3-3 protein
 MOD_RES 2 2 N-acetylthreonine; in 14-3-3 protein
 MOD_RES 60 60 Phosphoserine (By similarity).
 MOD_RES 70 70 N6-acetyllysine.
 MOD_RES 84 84 Nitrated tyrosine (By similarity).
 MOD_RES 106 106 Nitrated tyrosine (By similarity).
 MOD_RES 117 117 N6-acetyllysine.
 MOD_RES 186 186 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative initiation; Complete proteome; Cytoplasm; Direct protein sequencing; Nitration; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 246 AA 
Protein Sequence
MDKSELVQKA KLAEQAERYD DMAAAMKAVT EQGHELSNEE RNLLSVAYKN VVGARRSSWR 60
VISSIEQKTE RNEKKQQMGK EYREKIEAEL QDICNDVLEL LDKYLIPNAT QPESKVFYLK 120
MKGDYFRYLS EVASGDNKQT TVSNSQQAYQ EAFEISKKEM QPTHPIRLGL ALNFSVFYYE 180
ILNSPEKACS LAKTAFDEAI AELDTLNEES YKDSTLIMQL LRDNLTLWTS ENQGDEGDAG 240
EGEN 244 
Gene Ontology
 GO:0030659; C:cytoplasmic vesicle membrane; TAS:Reactome.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0017053; C:transcriptional repressor complex; IEA:Compara.
 GO:0003714; F:transcription corepressor activity; IEA:Compara.
 GO:0000186; P:activation of MAPKK activity; TAS:Reactome.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0051220; P:cytoplasmic sequestering of protein; IDA:BHF-UCL.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0035329; P:hippo signaling cascade; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0097193; P:intrinsic apoptotic signaling pathway; TAS:Reactome.
 GO:0000165; P:MAPK cascade; TAS:Reactome.
 GO:0016071; P:mRNA metabolic process; TAS:Reactome.
 GO:0035308; P:negative regulation of protein dephosphorylation; IDA:BHF-UCL.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:1900740; P:positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; TAS:Reactome.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0006605; P:protein targeting; IEA:Compara.
 GO:0007265; P:Ras protein signal transduction; TAS:Reactome. 
Interpro
 IPR000308; 14-3-3.
 IPR023409; 14-3-3_CS.
 IPR023410; 14-3-3_domain. 
Pfam
 PF00244; 14-3-3 
SMART
 SM00101; 14_3_3 
PROSITE
 PS00796; 1433_1
 PS00797; 1433_2 
PRINTS
 PR00305; 1433ZETA.