CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007420
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SIT4-associating protein SAP185 
Protein Synonyms/Alias
  
Gene Name
 SAP185 
Gene Synonyms/Alias
 YJL098W; J0840 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
20GSQSPLAKLLNRAFIubiquitination[1, 2]
32AFIKIDDKPTSTEAGubiquitination[2]
146DLVRGEDKDTTEDFEubiquitination[2]
169IDGTFEEKERTRSGEubiquitination[2]
708NECIEYEKGHDTRLGubiquitination[3]
874YVNEDGTKTRLNFNPubiquitination[2]
912RSFTDACKSETIPNNubiquitination[2]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Associates with the SIT4 phosphatase in a cell cycle dependent manner. May be directly or indirectly involved in SIT4- dependent functions in budding and in normal G1 cyclin expression. 
Sequence Annotation
  
Keyword
 Cell cycle; Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1058 AA 
Protein Sequence
MSGSFWKFGQ DFGSQSPLAK LLNRAFIKID DKPTSTEAGK IDSNSTDESL ESNSFKSEDE 60
EEEYELPNRE EDYKAYKPNL SLLNDLLDDE ELYTELMCSN FKLLVYLKYP EVLSKLIDYV 120
RNSTILESNI DRVTSEDRDL VRGEDKDTTE DFENAKADKK NIDGTFEEKE RTRSGEEEEL 180
ENEENDSASE DTRVTLPHEL EEHDDTRRAR IAAEILSADV WPISSALIEN EGLLAKLWSI 240
LRLPSPLSIE ASTYFMKINE RLLDMNMDGI IEFILKKEHI VDDFLAHIDN PPLMDFLLKV 300
ISTDKPEISN GVIQLFKKQN LVPKLIHLLD PVFDSCTQSA AGDFLKALVT ISGNCPNEIT 360
SSIGPNELTR QLVSPNMMKQ LMDIMLKGGN SLNNGVGIII ELIRKNNSDY DTIQTNYTTI 420
ESHPPTDRDP IYLGYLVKMF SEHMADFNKI LTEKKIPLLQ TSYGTIEPLG FERFKICELI 480
AELLHCSNMT LLNEPSAYDI VRERDAERER IFNSQNYVDS NDRSELKENE DDNTGDADDE 540
VEDDTNQVES ANTSIDGEEV IDKLNSLQIE TNKVNQNMNN EEQHSLMPDF NNGDFKDEED 600
ENPFEPQYSD VILDSSDIEK NFRVSPNVGD QLKISLQDTR VIDTMLEMFF HFQWNNFLHN 660
VVYDVVQQIF NGPLKIGYNR FLLDDLLINI RLTDMIINGN NECIEYEKGH DTRLGYMGHL 720
TLIAEEVTKF TAYIEEMNIT FENTEVMSSL FESKWIAYTE DVLEDLKEKY NAILGDIAEE 780
GDMLQDEEED AVYDKGERTM GTVDDYINDI MQMDNVRCQE EEEDEGEGYV SFDEDEPQEY 840
RNGDSVRSKE SNSSEGKRDQ EQLYYEYVNE DGTKTRLNFN PDSDATEQVP GEVNRDHKIP 900
LKLKRSFTDA CKSETIPNNT VNAKEESVFQ FSNELSDGWE SSPSNSIPKR ASPSKNGMNS 960
PMFQHQFELH SPTDEFGGHK DEILSAEGHD YDIDEYDELS DDSDEEYDNC EDEDSLDYAD 1020
SAAYALCRSK SKDKISWDEE EQARLMGVVK FNSEHYRD 1058 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0004722; F:protein serine/threonine phosphatase activity; IPI:SGD.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IPI:SGD.
 GO:0002098; P:tRNA wobble uridine modification; IGI:SGD. 
Interpro
 IPR007587; SAPS. 
Pfam
 PF04499; SAPS 
SMART
  
PROSITE
  
PRINTS