CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000320
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PDZ domain-containing protein GIPC1 
Protein Synonyms/Alias
 GAIP C-terminus-interacting protein; RGS-GAIP-interacting protein; RGS19-interacting protein 1; Tax interaction protein 2; TIP-2 
Gene Name
 GIPC1 
Gene Synonyms/Alias
 C19orf3; GIPC; RGS19IP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10LGLGRRKKAPPLVENubiquitination[1, 2]
80IEGFTNVKELYGKIAubiquitination[1, 2, 3, 4, 5, 6, 7]
128DFIFAHVKGQRKEVEubiquitination[1, 5]
199YEVARLLKELPRGRTubiquitination[5]
210RGRTFTLKLTEPRKAubiquitination[1, 5]
216LKLTEPRKAFDMISQubiquitination[4, 5]
263LPSAFEEKAIEKVDDubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 May be involved in G protein-linked signaling. 
Sequence Annotation
 DOMAIN 133 213 PDZ.
 MOD_RES 225 225 Phosphoserine.
 MOD_RES 232 232 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 333 AA 
Protein Sequence
MPLGLGRRKK APPLVENEEA EPGRGGLGVG EPGPLGGGGS GGPQMGLPPP PPALRPRLVF 60
HTQLAHGSPT GRIEGFTNVK ELYGKIAEAF RLPTAEVMFC TLNTHKVDMD KLLGGQIGLE 120
DFIFAHVKGQ RKEVEVFKSE DALGLTITDN GAGYAFIKRI KEGSVIDHIH LISVGDMIEA 180
INGQSLLGCR HYEVARLLKE LPRGRTFTLK LTEPRKAFDM ISQRSAGGRP GSGPQLGTGR 240
GTLRLRSRGP ATVEDLPSAF EEKAIEKVDD LLESYMGIRD TELAATMVEL GKDKRNPDEL 300
AEALDERLGD FAFPDEFVFD VWGAIGDAKV GRY 333 
Gene Ontology
 GO:0005903; C:brush border; IEA:Compara.
 GO:0005938; C:cell cortex; IDA:UniProtKB.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0043198; C:dendritic shaft; ISS:BHF-UCL.
 GO:0043197; C:dendritic spine; ISS:BHF-UCL.
 GO:0030139; C:endocytic vesicle; IEA:Compara.
 GO:0008021; C:synaptic vesicle; ISS:BHF-UCL.
 GO:0012506; C:vesicle membrane; ISS:BHF-UCL.
 GO:0003779; F:actin binding; ISS:BHF-UCL.
 GO:0017022; F:myosin binding; ISS:BHF-UCL.
 GO:0042803; F:protein homodimerization activity; ISS:BHF-UCL.
 GO:0043542; P:endothelial cell migration; ISS:BHF-UCL.
 GO:0007186; P:G-protein coupled receptor signaling pathway; NAS:UniProtKB.
 GO:0014047; P:glutamate secretion; ISS:BHF-UCL.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:BHF-UCL.
 GO:0030511; P:positive regulation of transforming growth factor beta receptor signaling pathway; ISS:BHF-UCL.
 GO:0006605; P:protein targeting; ISS:BHF-UCL.
 GO:0031647; P:regulation of protein stability; ISS:BHF-UCL.
 GO:0048167; P:regulation of synaptic plasticity; ISS:BHF-UCL.
 GO:0007268; P:synaptic transmission; ISS:BHF-UCL. 
Interpro
 IPR001478; PDZ.
 IPR017379; UCP038083_PDZ. 
Pfam
 PF00595; PDZ 
SMART
 SM00228; PDZ 
PROSITE
 PS50106; PDZ 
PRINTS