CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023724
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Suppressor of G2 allele of SKP1 homolog 
Protein Synonyms/Alias
 Protein 40-6-3; Sgt1 
Gene Name
 SUGT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
41LTKALEQKPDDAQYYubiquitination[1]
256PEAVRWEKLEGQGDVubiquitination[2, 3]
267QGDVPTPKQFVADVKubiquitination[2, 3]
274KQFVADVKNLYPSSSubiquitination[2, 3]
302KEEEKNEKLEGDAALubiquitination[4]
325SDGSDEVKRAMNKSFubiquitination[1, 4]
349TNWSDVGKRKVEINPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 May play a role in ubiquitination and subsequent proteasomal degradation of target proteins. 
Sequence Annotation
 REPEAT 11 44 TPR 1.
 REPEAT 45 78 TPR 2.
 REPEAT 79 112 TPR 3.
 DOMAIN 169 258 CS.
 DOMAIN 276 365 SGS.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 265 265 Phosphothreonine.
 MOD_RES 281 281 Phosphoserine.
 MOD_RES 331 331 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 365 AA 
Protein Sequence
MAAAAAGTAT SQRFFQSFSD ALIDEDPQAA LEELTKALEQ KPDDAQYYCQ RAYCHILLGN 60
YCVAVADAKK SLELNPNNST AMLRKGICEY HEKNYAAALE TFTEGQKLDI ETGFHRVGQA 120
GLQLLTSSDP PALDSQSAGI TGADANFSVW IKRCQEAQNG SESEVWTHQS KIKYDWYQTE 180
SQVVITLMIK NVQKNDVNVE FSEKELSALV KLPSGEDYNL KLELLHPIIP EQSTFKVLST 240
KIEIKLKKPE AVRWEKLEGQ GDVPTPKQFV ADVKNLYPSS SPYTRNWDKL VGEIKEEEKN 300
EKLEGDAALN RLFQQIYSDG SDEVKRAMNK SFMESGGTVL STNWSDVGKR KVEINPPDDM 360
EWKKY 365 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000776; C:kinetochore; TAS:ProtInc.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000151; C:ubiquitin ligase complex; TAS:ProtInc.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0035872; P:nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR007052; CS-like_domain.
 IPR017447; CS_domain.
 IPR008978; HSP20-like_chaperone.
 IPR007699; SGS.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF04969; CS
 PF05002; SGS 
SMART
  
PROSITE
 PS51203; CS
 PS51048; SGS
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS