CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022965
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Prefoldin subunit 2 
Protein Synonyms/Alias
  
Gene Name
 PFDN2 
Gene Synonyms/Alias
 PFD2; HSPC231 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18SSGSGAGKGAVSAEQubiquitination[1, 2, 3, 4, 5, 6]
43EQRGLASKAAELEMEubiquitination[3]
94LPALENNKEQIQKIIubiquitination[2, 4, 5, 6]
99NNKEQIQKIIETLTQubiquitination[2, 5, 6, 7]
111LTQQLQAKGKELNEFubiquitination[2, 4]
113QQLQAKGKELNEFREubiquitination[4, 6]
132RLMGEDEKPAAKENSacetylation[6]
132RLMGEDEKPAAKENSubiquitination[2, 3, 4, 6]
136EDEKPAAKENSEGAGubiquitination[2, 4, 6]
145NSEGAGAKASSAGVLubiquitination[2, 4, 5, 6, 8]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. 
Sequence Annotation
  
Keyword
 Chaperone; Complete proteome; Cytoplasm; Mitochondrion; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 154 AA 
Protein Sequence
MAENSGRAGK SSGSGAGKGA VSAEQVIAGF NRLRQEQRGL ASKAAELEME LNEHSLVIDT 60
LKEVDETRKC YRMVGGVLVE RTVKEVLPAL ENNKEQIQKI IETLTQQLQA KGKELNEFRE 120
KHNIRLMGED EKPAAKENSE GAGAKASSAG VLVS 154 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0016272; C:prefoldin complex; NAS:UniProtKB.
 GO:0051082; F:unfolded protein binding; NAS:UniProtKB.
 GO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome. 
Interpro
 IPR027235; PFD2.
 IPR002777; PFD_beta-like.
 IPR009053; Prefoldin. 
Pfam
 PF01920; Prefoldin_2 
SMART
  
PROSITE
  
PRINTS