UniProt Accession

 KAD2_YEAST; P07170; D6VSK8; Q6Q539 

Genbank Protein ID

 X06304; Y00413; M18455; U13239; Z48612; AY558578; BK006938 

Genbank Nucleotide ID

 CAA29624.1; CAA68471.1; AAA66319.1; AAC33143.1; CAA88506.1; AAS56904.1; DAA12068.1 

Protein Name

 Adenylate kinase 

Protein Synonyms/Alias

 ATP-AMP transphosphorylase; ATP:AMP phosphotransferase; Adenylate kinase cytosolic and mitochondrial; Adenylate monophosphate kinase 

Gene Name

 ADK1 

Gene Synonyms/Alias

 AKY; AKY1; AKY2; YDR226W; YD9934.11 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
19	IGPPGAGKGTQAPNL	acetylation	[1]
47	MLRSQIAKGTQLGLE	acetylation	[1]
47	MLRSQIAKGTQLGLE	ubiquitination	[2]
102	RTIPQAEKLDQMLKE	acetylation	[1]
108	EKLDQMLKEQGTPLE	acetylation	[1]
116	EQGTPLEKAIELKVD	acetylation	[1]
116	EQGTPLEKAIELKVD	ubiquitination	[2]
146	ASGRSYHKIFNPPKE	acetylation	[1]
156	NPPKEDMKDDVTGEA	acetylation	[1]
176	DDNADALKKRLAAYH	acetylation	[1]
194	EPIVDFYKKTGIWAG	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. 

Sequence Annotation

 NP_BIND 16 21 ATP.
 NP_BIND 63 65 AMP.
 NP_BIND 92 95 AMP.
 NP_BIND 143 144 ATP.
 REGION 36 65 NMPbind.
 REGION 133 170 LID.
 BINDING 37 37 AMP.
 BINDING 42 42 AMP.
 BINDING 99 99 AMP.
 BINDING 134 134 ATP.
 BINDING 167 167 AMP.
 BINDING 178 178 AMP.
 BINDING 206 206 ATP; via carbonyl oxygen.
 MOD_RES 3 3 N-acetylserine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Mitochondrion; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 222 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
 GO:0004017; F:adenylate kinase activity; IMP:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006172; P:ADP biosynthetic process; IDA:SGD.
 GO:0006270; P:DNA replication initiation; IMP:SGD. 

Interpro

 IPR006259; Adenyl_kin_sub.
 IPR000850; Adenylate_kin.
 IPR007862; Adenylate_kinase_lid-dom.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00406; ADK
 PF05191; ADK_lid 

SMART

  

PROSITE

 PS00113; ADENYLATE_KINASE 

PRINTS

 PR00094; ADENYLTKNASE.