CPLM-021283

Genbank Nucleotide ID

DNA replication factor Cdt1

Protein Synonyms/Alias

Double parked homolog; DUP

Homo sapiens (Human)

Position	Peptide	Type	References
24	PPRIAPPKLACRTPS	acetylation	[1]
24	PPRIAPPKLACRTPS	ubiquitination	[2, 3]
49	SATSGSRKRARPPAA	acetylation	[1]
141	GARVRALKASAQDAG	ubiquitination	[2, 4, 5]
166	PEEPCGEKAPAYQRF	ubiquitination	[2, 4]
189	PGLVLPYKYQVLAEM	ubiquitination	[6]
218	SETPTFAKVQRGVQD	ubiquitination	[2]
240	ECNVGQIKTVYPASY	ubiquitination	[7, 8]
259	ERSVPTFKDGTRRSD	ubiquitination	[2]
301	RRQIFSQKLVEHVKE	ubiquitination	[2]
307	QKLVEHVKEHHKAFL	ubiquitination	[8]
356	PQPPATEKLTTAQEV	ubiquitination	[2, 6, 8]
377	LISPRMEKALSQLAL	ubiquitination	[2, 8]
416	AASPSALKGVSQDLL	ubiquitination	[2, 8]
433	IRAKEAQKQLAQMTR	ubiquitination	[2, 8]
470	SVFVSERKPALSMEV	ubiquitination	[2]
522	IRTDTYVKLDKAADL	ubiquitination	[2, 8]
525	DTYVKLDKAADLAHI	ubiquitination	[2]

[1] Acetylation/deacetylation modulates the stability of DNA replication licensing factor Cdt1.
Glozak MA, Seto E.
J Biol Chem. 2009 Apr 24;284(17):11446-53. [PMID: 19276081]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Cooperates with CDC6 to promote the loading of the mini- chromosome maintenance complex onto chromatin to form the pre- replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.

REGION 150 190 Interaction with GMNN.
MOTIF 1 23 PIP-box K+4 motif.
MOTIF 68 70 Cyclin-binding motif.
MOD_RES 29 29 Phosphothreonine; by MAPK8.
MOD_RES 318 318 Phosphoserine.
MOD_RES 394 394 Phosphoserine.

3D-structure; Cell cycle; Complete proteome; Disease mutation; DNA replication; DNA-binding; Dwarfism; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0003677; F:DNA binding; ISS:UniProtKB.
GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
GO:0000076; P:DNA replication checkpoint; IDA:UniProtKB.
GO:0030174; P:regulation of DNA-dependent DNA replication initiation; IDA:UniProtKB.
GO:0000083; P:regulation of transcription involved in G1/S phase of mitotic cell cycle; TAS:Reactome.

IPR014939; CDT1_Gemini-bd-like.