| Tag | Content |
|---|
| CPLM ID | CPLM-004278 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | General transcriptional corepressor CYC8 |
Protein Synonyms/Alias | Glucose repression mediator protein CYC8 |
Gene Name | CYC8 |
Gene Synonyms/Alias | CRT8; SSN6; YBR112C; YBR0908 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 175 | YAEEAFAKVLELDPH | ubiquitination | [1] | | 738 | EPQVKKQKLNSPNSN | acetylation | [2] | | 748 | SPNSNINKLVNTATS | acetylation | [2] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex. |
Sequence Annotation | REPEAT 46 79 TPR 1.
REPEAT 80 113 TPR 2.
REPEAT 114 147 TPR 3.
REPEAT 150 183 TPR 4.
REPEAT 187 220 TPR 5.
REPEAT 224 257 TPR 6.
REPEAT 262 295 TPR 7.
REPEAT 296 329 TPR 8.
REPEAT 330 363 TPR 9.
REPEAT 364 398 TPR 10.
REGION 467 682 Prion domain (PrD).
MOD_RES 429 429 Phosphoserine.
MOD_RES 475 475 Phosphothreonine.
MOD_RES 710 710 Phosphoserine.
MOD_RES 741 741 Phosphoserine.
MOD_RES 768 768 Phosphoserine.
MOD_RES 780 780 Phosphoserine; by ATM or ATR.
MOD_RES 815 815 Phosphoserine.
MOD_RES 817 817 Phosphoserine.
MOD_RES 866 866 Phosphoserine.
MOD_RES 943 943 Phosphoserine. |
Keyword | Amyloid; Complete proteome; Nucleus; Phosphoprotein; Prion; Reference proteome; Repeat; Repressor; TPR repeat; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 966 AA |
Protein Sequence | MNPGGEQTIM EQPAQQQQQQ QQQQQQQQQQ AAVPQQPLDP LTQSTAETWL SIASLAETLG 60
DGDRAAMAYD ATLQFNPSSA KALTSLAHLY RSRDMFQRAA ELYERALLVN PELSDVWATL 120
GHCYLMLDDL QRAYNAYQQA LYHLSNPNVP KLWHGIGILY DRYGSLDYAE EAFAKVLELD 180
PHFEKANEIY FRLGIIYKHQ GKWSQALECF RYILPQPPAP LQEWDIWFQL GSVLESMGEW 240
QGAKEAYEHV LAQNQHHAKV LQQLGCLYGM SNVQFYDPQK ALDYLLKSLE ADPSDATTWY 300
HLGRVHMIRT DYTAAYDAFQ QAVNRDSRNP IFWCSIGVLY YQISQYRDAL DAYTRAIRLN 360
PYISEVWYDL GTLYETCNNQ LSDALDAYKQ AARLDVNNVH IRERLEALTK QLENPGNINK 420
SNGAPTNASP APPPVILQPT LQPNDQGNPL NTRISAQSAN ATASMVQQQH PAQQTPINSS 480
ATMYSNGASP QLQAQAQAQA QAQAQAQAQA QAQAQAQAQA QAQAQAQAQA QAQAQAHAQA 540
QAQAQAQAQA QAQAQAQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQLQP LPRQQLQQKG 600
VSVQMLNPQQ GQPYITQPTV IQAHQLQPFS TQAMEHPQSS QLPPQQQQLQ SVQHPQQLQG 660
QPQAQAPQPL IQHNVEQNVL PQKRYMEGAI HTLVDAAVSS STHTENNTKS PRQPTHAIPT 720
QAPATGITNA EPQVKKQKLN SPNSNINKLV NTATSIEENA KSEVSNQSPA VVESNTNNTS 780
QEEKPVKANS IPSVIGAQEP PQEASPAEEA TKAASVSPST KPLNTEPESS SVQPTVSSES 840
STTKANDQST AETIELSTAT VPAEASPVED EVRQHSKEEN GTTEASAPST EEAEPAASRD 900
AEKQQDETAA TTITVIKPTL ETMETVKEEA KMREEEQTSQ EKSPQENTLP RENVVRQVEE 960
DENYDD 966 |
Gene Ontology | GO:0017053; C:transcriptional repressor complex; IDA:SGD. GO:0042826; F:histone deacetylase binding; IDA:SGD. GO:0001191; F:RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription; IDA:SGD. GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; IDA:SGD. GO:0006338; P:chromatin remodeling; IGI:SGD. GO:0035955; P:negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter; IMP:SGD. GO:0007070; P:negative regulation of transcription from RNA polymerase II promoter during mitosis; IMP:SGD. GO:0016584; P:nucleosome positioning; IDA:SGD. GO:2000531; P:regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter; IMP:SGD. GO:2001020; P:regulation of response to DNA damage stimulus; IMP:SGD. GO:0061392; P:regulation of transcription from RNA polymerase II promoter in response to osmotic stress; IMP:SGD. |
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