CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031091
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine hydroxymethyltransferase 
Protein Synonyms/Alias
  
Gene Name
 SHMT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21GFMTDKKKISATSIFubiquitination[1]
35FFESMPYKVNPDTGYubiquitination[1, 2, 3, 4]
58NARLFHPKLIIAGTSubiquitination[1]
237TDGGRAEKVLEACSIacetylation[5]
237TDGGRAEKVLEACSIubiquitination[1]
248ACSIACNKNTCPGDRubiquitination[1, 3, 5, 6]
278TSRGLLEKDFQKVAHubiquitination[1, 2, 4, 5, 6, 7]
282LLEKDFQKVAHFIHRubiquitination[1, 7]
308TGVRATLKEFKERLAubiquitination[1, 5]
311RATLKEFKERLAGDKubiquitination[1]
318KERLAGDKYQAAVQAubiquitination[1, 2, 3, 5, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
 Interconversion of serine and glycine (By similarity). 
Sequence Annotation
  
Keyword
 Complete proteome; Methyltransferase; One-carbon metabolism; Pyridoxal phosphate; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 345 AA 
Protein Sequence
MGLDLPDGGH LTHGFMTDKK KISATSIFFE SMPYKVNPDT GYINYDQLEE NARLFHPKLI 60
IAGTSCYSRN LEYARLRKIA DENGAYLMAD MAHISGLVAA GVVPSPFEHC HVVTTTTHKT 120
LRGCRAGMIF YRKGVKSVDP KTGKEILYNL ESLINSAVFP GLQGGPHNHA IAGVAVALKQ 180
AMTLEFKVYQ HQVVANCRAL SEALTELGYK IVTGGSDNHL ILVDLRSKGT DGGRAEKVLE 240
ACSIACNKNT CPGDRSALRP SGLRLGTPAL TSRGLLEKDF QKVAHFIHRG IELTLQIQSD 300
TGVRATLKEF KERLAGDKYQ AAVQALREEV ESFASLFPLP GLPDF 345 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0016597; F:amino acid binding; IEA:Compara.
 GO:0004372; F:glycine hydroxymethyltransferase activity; IEA:EC.
 GO:0008732; F:L-allo-threonine aldolase activity; IEA:Compara.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0019264; P:glycine biosynthetic process from serine; IEA:Compara.
 GO:0051289; P:protein homotetramerization; IEA:Compara.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 
Interpro
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2.
 IPR001085; Ser_HO-MeTrfase.
 IPR019798; Ser_HO-MeTrfase_PLP_BS. 
Pfam
 PF00464; SHMT 
SMART
  
PROSITE
 PS00096; SHMT 
PRINTS