CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036252
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoribosylaminoimidazole carboxylase 
Protein Synonyms/Alias
 Phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase, isoform CRA_b 
Gene Name
 PAICS 
Gene Synonyms/Alias
 hCG_2027161 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18IATGSFLKRNPGVKEacetylation[1]
18IATGSFLKRNPGVKEubiquitination[1, 2, 3, 4, 5]
24LKRNPGVKEGYKFYPubiquitination[1, 3, 5]
33GYKFYPPKVELFFKDubiquitination[2]
39PKVELFFKDDANNDPubiquitination[3, 6, 7]
143KQSYRDLKEVTPEGLacetylation[8]
143KQSYRDLKEVTPEGLubiquitination[1, 3, 5, 7]
154PEGLQMVKKNFEWVAacetylation[1, 8, 9]
154PEGLQMVKKNFEWVAubiquitination[3, 4, 5, 7]
155EGLQMVKKNFEWVAEacetylation[1]
155EGLQMVKKNFEWVAEubiquitination[1, 3, 5]
169ERVELLLKSESQCRVacetylation[1]
169ERVELLLKSESQCRVubiquitination[5, 6]
191SDLGHCEKIKKACGNacetylation[1, 8]
191SDLGHCEKIKKACGNubiquitination[5]
193LGHCEKIKKACGNFGacetylation[1]
193LGHCEKIKKACGNFGubiquitination[5]
194GHCEKIKKACGNFGIubiquitination[1, 4, 5]
212LRVTSAHKGPDETLRubiquitination[1, 3, 5, 6, 7]
221PDETLRIKAEYEGDGubiquitination[3, 5, 10]
322LNTWISLKQADKKIRubiquitination[1, 3, 5]
326ISLKQADKKIRECNLubiquitination[6]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 333 AA 
Protein Sequence
MIPIEWVCRR IATGSFLKRN PGVKEGYKFY PPKVELFFKD DANNDPQWSE EQLIAAKFCF 60
AGLLIGQTEV DIMSHATQAI FEILEKSWLP QNCTLVDMKI EFGVDVTTKE IVLADVIDND 120
SWRLWPSGDR SQQKDKQSYR DLKEVTPEGL QMVKKNFEWV AERVELLLKS ESQCRVVVLM 180
GSTSDLGHCE KIKKACGNFG IPCELRVTSA HKGPDETLRI KAEYEGDGIP TVFVAVAGRS 240
NGLGPVMSGN TAYPVISCPP LTPDWGVQDV WSSLRLPSGL GCSTVLSPEG SAQFAAQIFG 300
LSNHLVWSKL RASILNTWIS LKQADKKIRE CNL 333 
Gene Ontology
 GO:0034023; F:5-(carboxyamino)imidazole ribonucleotide mutase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IEA:InterPro.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:InterPro. 
Interpro
 IPR013816; ATP_grasp_subdomain_2.
 IPR000031; N5-CAIR_Mutase_PurE_dom.
 IPR001636; SAICAR_synth.
 IPR018236; SAICAR_synthetase_CS. 
Pfam
 PF00731; AIRC
 PF01259; SAICAR_synt 
SMART
 SM01001; AIRC 
PROSITE
 PS01057; SAICAR_SYNTHETASE_1
 PS01058; SAICAR_SYNTHETASE_2 
PRINTS