CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012119
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Baculoviral IAP repeat-containing protein 3 
Protein Synonyms/Alias
 Apoptosis inhibitor 2; API2; C-IAP2; IAP homolog C; Inhibitor of apoptosis protein 1; IAP-1; hIAP-1; hIAP1; RING finger protein 49; TNFR2-TRAF-signaling complex protein 1 
Gene Name
 BIRC3 
Gene Synonyms/Alias
 API2; IAP1; MIHC; RNF49 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
63YYTGVNDKVKCFCCGubiquitination[1]
211ACFACGGKLSNWEPKubiquitination[1]
218KLSNWEPKDNAMSEHubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase- independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. 
Sequence Annotation
 REPEAT 29 96 BIR 1.
 REPEAT 169 235 BIR 2.
 REPEAT 255 322 BIR 3.
 DOMAIN 439 529 CARD.
 ZN_FING 557 592 RING-type.
 METAL 292 292 Zinc (By similarity).
 METAL 295 295 Zinc (By similarity).
 METAL 312 312 Zinc (By similarity).
 METAL 319 319 Zinc (By similarity).  
Keyword
 3D-structure; Apoptosis; Chromosomal rearrangement; Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 604 AA 
Protein Sequence
MNIVENSIFL SNLMKSANTF ELKYDLSCEL YRMSTYSTFP AGVPVSERSL ARAGFYYTGV 60
NDKVKCFCCG LMLDNWKRGD SPTEKHKKLY PSCRFVQSLN SVNNLEATSQ PTFPSSVTNS 120
THSLLPGTEN SGYFRGSYSN SPSNPVNSRA NQDFSALMRS SYHCAMNNEN ARLLTFQTWP 180
LTFLSPTDLA KAGFYYIGPG DRVACFACGG KLSNWEPKDN AMSEHLRHFP KCPFIENQLQ 240
DTSRYTVSNL SMQTHAARFK TFFNWPSSVL VNPEQLASAG FYYVGNSDDV KCFCCDGGLR 300
CWESGDDPWV QHAKWFPRCE YLIRIKGQEF IRQVQASYPH LLEQLLSTSD SPGDENAESS 360
IIHFEPGEDH SEDAIMMNTP VINAAVEMGF SRSLVKQTVQ RKILATGENY RLVNDLVLDL 420
LNAEDEIREE ERERATEEKE SNDLLLIRKN RMALFQHLTC VIPILDSLLT AGIINEQEHD 480
VIKQKTQTSL QARELIDTIL VKGNIAATVF RNSLQEAEAV LYEHLFVQQD IKYIPTEDVS 540
DLPVEEQLRR LQEERTCKVC MDKEVSIVFI PCGHLVVCKD CAPSLRKCPI CRSTIKGTVR 600
TFLS 604 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007166; P:cell surface receptor signaling pathway; TAS:ProtInc.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0070266; P:necroptosis; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; TAS:ProtInc.
 GO:0060546; P:negative regulation of necroptosis; IEA:Compara.
 GO:0042326; P:negative regulation of phosphorylation; IEA:Compara.
 GO:2000378; P:negative regulation of reactive oxygen species metabolic process; IEA:Compara.
 GO:0038061; P:NIK/NF-kappaB cascade; TAS:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; TAS:UniProtKB.
 GO:2000116; P:regulation of cysteine-type endopeptidase activity; TAS:UniProtKB.
 GO:0050727; P:regulation of inflammatory response; TAS:UniProtKB.
 GO:0070424; P:regulation of nucleotide-binding oligomerization domain containing signaling pathway; TAS:UniProtKB.
 GO:0039535; P:regulation of RIG-I signaling pathway; TAS:UniProtKB.
 GO:0034121; P:regulation of toll-like receptor signaling pathway; TAS:UniProtKB.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR001370; BIR.
 IPR001315; CARD.
 IPR011029; DEATH-like_dom.
 IPR001841; Znf_RING. 
Pfam
 PF00653; BIR
 PF00619; CARD 
SMART
 SM00238; BIR
 SM00114; CARD
 SM00184; RING 
PROSITE
 PS01282; BIR_REPEAT_1
 PS50143; BIR_REPEAT_2
 PS50209; CARD
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS