UniProt Accession

 APPY_ECOLI; P05052; Q2MBL7 

Genbank Protein ID

 Y00138; M24530; U82598; U00096; AP009048 

Genbank Nucleotide ID

 CAA68331.1; AAA23454.1; AAB40760.1; AAC73665.1; BAE76339.1 

Protein Name

 HTH-type transcriptional regulator AppY 

Protein Synonyms/Alias

 M5 polypeptide 

Gene Name

 appY 

Gene Synonyms/Alias

 b0564; JW0553 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
36	SLFIVSDKIRRELPV	acetylation	[1]
47	ELPVCPSKLRIVDID	acetylation	[1]
55	LRIVDIDKKTCLSFF	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Induces the synthesis of acid phosphatase (AppA) and several other polypeptides (such as AppBC) during the deceleration phase of growth. It also acts as a transcriptional repressor for one group of proteins that are synthesized preferentially in exponential growth and for one group synthesized only in the stationary phase. Also involved in the stabilization of the sigma stress factor RpoS during stress conditions. 

Sequence Annotation

 DNA_BIND 155 174 H-T-H motif (By similarity).  

Keyword

 Complete proteome; DNA-binding; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 249 AA 

Protein Sequence

Gene Ontology

 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:EcoCyc.
 GO:0016036; P:cellular response to phosphate starvation; IDA:EcoCyc.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:EcoCyc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR009057; Homeodomain-like.
 IPR018060; HTH_AraC.
 IPR018062; HTH_AraC-typ_CS.
 IPR020449; Tscrpt_reg_HTH_AraC-type. 

Pfam

 PF12833; HTH_18 

SMART

 SM00342; HTH_ARAC 

PROSITE

 PS00041; HTH_ARAC_FAMILY_1
 PS01124; HTH_ARAC_FAMILY_2 

PRINTS

 PR00032; HTHARAC.