UniProt Accession

 PPM1A_HUMAN; P35813; B5BU11; J3KNM0; O75551 

Genbank Protein ID

 S87759; AF070670; AK097843; AB451247; AL132778; AL157756; CH471061; BC026691; BC063243 

Genbank Nucleotide ID

 AAB21784.1; AAC28354.1; BAG70061.1; EAW80774.1; AAH26691.1; AAH63243.1 

Protein Name

 Protein phosphatase 1A 

Protein Synonyms/Alias

 Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA 

Gene Name

 PPM1A 

Gene Synonyms/Alias

 PPPM1A 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
98	APSVENVKNGIRTGF	ubiquitination	[1]
203	ALGDFDYKCVHGKGP	ubiquitination	[1, 2]
278	VVDTCLYKGSRDNMS	ubiquitination	[1]
310	KKEAELDKYLECRVE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. 

Sequence Annotation

 METAL 60 60 Manganese 1.
 METAL 60 60 Manganese 2.
 METAL 61 61 Manganese 1; via carbonyl oxygen.
 METAL 239 239 Manganese 2.
 METAL 282 282 Manganese 2.
 MOD_RES 375 375 Phosphoserine.
 MOD_RES 377 377 Phosphoserine (By similarity).
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Hydrolase; Lipoprotein; Magnesium; Manganese; Metal-binding; Myristate; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 382 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0016020; C:membrane; ISS:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0033192; F:calmodulin-dependent protein phosphatase activity; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030145; F:manganese ion binding; IEA:InterPro.
 GO:0004871; F:signal transducer activity; IMP:UniProtKB.
 GO:0007050; P:cell cycle arrest; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0006499; P:N-terminal protein myristoylation; ISS:UniProtKB.
 GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0042347; P:negative regulation of NF-kappaB import into nucleus; IMP:UniProtKB.
 GO:0010991; P:negative regulation of SMAD protein complex assembly; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:Reactome.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:UniProtKB.
 GO:0035970; P:peptidyl-threonine dephosphorylation; IDA:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:BHF-UCL.
 GO:0030177; P:positive regulation of Wnt receptor signaling pathway; IDA:BHF-UCL.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome.
 GO:0016055; P:Wnt receptor signaling pathway; IDA:BHF-UCL. 

Interpro

 IPR001932; PP2C-like.
 IPR012911; PP2C_C.
 IPR000222; PP2C_Mn2_Asp60_BS.
 IPR015655; Protein_Pase_2C. 

Pfam

 PF00481; PP2C
 PF07830; PP2C_C 

SMART

 SM00331; PP2C_SIG
 SM00332; PP2Cc 

PROSITE

 PS01032; PP2C 

PRINTS