UniProt Accession

 MYL6B_HUMAN; P14649 

Genbank Protein ID

 X16434; M31211; BC012425; BC014400 

Genbank Nucleotide ID

 CAA34457.1; AAA36320.1; AAH12425.1; AAH14400.1 

Protein Name

 Myosin light chain 6B 

Protein Synonyms/Alias

 Myosin light chain 1 slow-twitch muscle A isoform; MLC1sa; Smooth muscle and nonmuscle myosin light chain alkali 6B 

Gene Name

 MYL6B 

Gene Synonyms/Alias

 MLC1SA 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
107	PTNAEVLKVLGNPKS	acetylation	[1]
107	PTNAEVLKVLGNPKS	ubiquitination	[2, 3, 4, 5, 6]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Regulatory light chain of myosin. Does not bind calcium. 

Sequence Annotation

 DOMAIN 64 99 EF-hand 1.
 DOMAIN 141 176 EF-hand 2.
 DOMAIN 176 208 EF-hand 3.  

Keyword

 3D-structure; Complete proteome; Motor protein; Muscle protein; Myosin; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 208 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005859; C:muscle myosin complex; TAS:ProtInc.
 GO:0016461; C:unconventional myosin complex; TAS:BHF-UCL.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003774; F:motor activity; TAS:HGNC.
 GO:0008307; F:structural constituent of muscle; TAS:HGNC.
 GO:0030049; P:muscle filament sliding; TAS:HGNC.
 GO:0007519; P:skeletal muscle tissue development; TAS:HGNC. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 

Pfam

 PF13405; EF_hand_4 

SMART

 SM00054; EFh 

PROSITE

 PS50222; EF_HAND_2 

PRINTS