CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016945
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rhophilin-2 
Protein Synonyms/Alias
 76 kDa RhoB effector protein; GTP-Rho-binding protein 2; p76RBE 
Gene Name
 RHPN2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
49ALNQQILKAVRMRTGubiquitination[1]
68LKVATNSKVREQVRLubiquitination[2]
441RSIEVLQKVLCAAQEubiquitination[1]
579SEVMKLLKSFGEDEIubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Binds specifically to GTP-Rho. May function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. 
Sequence Annotation
 REPEAT 38 110 REM.
 DOMAIN 111 460 BRO1.
 DOMAIN 515 593 PDZ.
 REGION 46 66 Interaction with Rho.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 686 AA 
Protein Sequence
MTDALLPAAP QPLEKENDGY FRKGCNPLAQ TGRSKLQNQR AALNQQILKA VRMRTGAENL 60
LKVATNSKVR EQVRLELSFV NSDLQMLKEE LEGLNISVGV YQNTEEAFTI PLIPLGLKET 120
KDVDFAVVLK DFILEHYSED GYLYEDEIAD LMDLRQACRT PSRDEAGVEL LMTYFIQLGF 180
VESRFFPPTR QMGLLFTWYD SLTGVPVSQQ NLLLEKASVL FNTGALYTQI GTRCDRQTQA 240
GLESAIDAFQ RAAGVLNYLK DTFTHTPSYD MSPAMLSVLV KMMLAQAQES VFEKISLPGI 300
RNEFFMLVKV AQEAAKVGEV YQQLHAAMSQ APVKENIPYS WASLACVKAH HYAALAHYFT 360
AILLIDHQVK PGTDLDHQEK CLSQLYDHMP EGLTPLATLK NDQQRRQLGK SHLRRAMAHH 420
EESVREASLC KKLRSIEVLQ KVLCAAQERS RLTYAQHQEE DDLLNLIDAP SVVAKTEQEV 480
DIILPQFSKL TVTDFFQKLG PLSVFSANKR WTPPRSIRFT AEEGDLGFTL RGNAPVQVHF 540
LDPYCSASVA GAREGDYIVS IQLVDCKWLT LSEVMKLLKS FGEDEIEMKV VSLLDSTSSM 600
HNKSATYSVG MQKTYSMICL AIDDDDKTDK TKKISKKLSF LSWGTNKNRQ KSASTLCLPS 660
VGAARPQVKK KLPSPFSLLN SDSSWY 686 
Gene Ontology
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR004328; BRO1_dom.
 IPR011072; HR1_rho-bd.
 IPR001478; PDZ. 
Pfam
 PF03097; BRO1
 PF02185; HR1 
SMART
 SM01041; BRO1
 SM00742; Hr1
 SM00228; PDZ 
PROSITE
 PS51180; BRO1
 PS50106; PDZ 
PRINTS