UniProt Accession

 RL9_HUMAN; P32969 

Genbank Protein ID

 D14531; U09953; U09954; U21138; AB062431; BC000483; BC004156; BC004206; BC007967; BC012149; BC031906; BC066318; BC070214; AB007169 

Genbank Nucleotide ID

 BAA03401.1; AAB01040.1; AAB01041.1; AAA63752.1; BAB93494.1; AAH00483.1; AAH04156.1; AAH04206.1; AAH07967.1; AAH12149.1; AAH31906.1; AAH66318.1; AAH70214.1; BAA25830.1 

Protein Name

 60S ribosomal protein L9 

Protein Synonyms/Alias

  

Gene Name

 RPL9; RPL9P7; RPL9P8; RPL9P9 

Gene Synonyms/Alias

 OK/SW-cl.103 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
2	******MKTILSNQT	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
21	ENVDITLKGRTVIVK	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
28	KGRTVIVKGPRGTLR	ubiquitination	[6, 7, 8]
50	VELSLLGKKKKRLRV	ubiquitination	[2, 3, 5, 8, 9]
51	ELSLLGKKKKRLRVD	ubiquitination	[7]
52	LSLLGKKKKRLRVDK	ubiquitination	[7]
59	KKRLRVDKWWGNRKE	ubiquitination	[7]
65	DKWWGNRKELATVRT	ubiquitination	[2, 6, 9]
82	SHVQNMIKGVTLGFR	ubiquitination	[5, 7]
121	IRNFLGEKYIRRVRM	acetylation	[10]
121	IRNFLGEKYIRRVRM	ubiquitination	[1, 2, 3, 5, 6, 7, 8, 9]
168	IQQATTVKNKDIRKF	ubiquitination	[3, 8]
174	VKNKDIRKFLDGIYV	ubiquitination	[1, 2, 3, 5, 6, 7, 9]
184	DGIYVSEKGTVQQAD	ubiquitination	[2, 3, 7, 8, 9]

Reference

 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

  

Sequence Annotation

 MOD_RES 121 121 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Reference proteome; Ribonucleoprotein; Ribosomal protein. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 192 AA 

Protein Sequence

Gene Ontology

 GO:0022625; C:cytosolic large ribosomal subunit; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0019843; F:rRNA binding; IEA:InterPro.
 GO:0003735; F:structural constituent of ribosome; NAS:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
 GO:0006414; P:translational elongation; TAS:Reactome.
 GO:0006413; P:translational initiation; TAS:Reactome.
 GO:0006415; P:translational termination; TAS:Reactome.
 GO:0019083; P:viral transcription; TAS:Reactome. 

Interpro

 IPR000702; Ribosomal_L6.
 IPR020040; Ribosomal_L6_a/b-dom.
 IPR002359; Ribosomal_L6_CS2. 

Pfam

 PF00347; Ribosomal_L6 

SMART

  

PROSITE

 PS00700; RIBOSOMAL_L6_2 

PRINTS