UniProt Accession

 ILVE_MYCS2; A0R066; I7FPI5 

Genbank Protein ID

 CP000480; CP001663 

Genbank Nucleotide ID

 ABK74679.1; AFP40633.1 

Protein Name

 Branched-chain-amino-acid aminotransferase 

Protein Synonyms/Alias

 BCAT 

Gene Name

 ilvE 

Gene Synonyms/Alias

 MSMEG_4276; MSMEI_4176 

Created Date

 July 27, 2013 

Organism

 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 

NCBI Taxa ID

 246196 

Lysine Modification

Position	Peptide	Type	References
299	IDVDEWQKKAGAGEI	pupylation	[1]

Reference

 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657] 

Functional Description

 Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to their efficiency as amino donor: Leu > Ile > Val. 

Sequence Annotation

 REGION 271 272 Pyridoxal phosphate binding.
 BINDING 101 101 Pyridoxal phosphate.
 BINDING 209 209 Pyridoxal phosphate.
 BINDING 314 314 Pyridoxal phosphate.
 MOD_RES 204 204 N6-(pyridoxal phosphate)lysine.
 CROSSLNK 299 299 Isoglutamyl lysine isopeptide (Lys-Gln)  

Keyword

 3D-structure; Amino-acid biosynthesis; Aminotransferase; Branched-chain amino acid biosynthesis; Complete proteome; Isopeptide bond; Pyridoxal phosphate; Reference proteome; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 368 AA 

Protein Sequence

Gene Ontology

 GO:0004084; F:branched-chain-amino-acid transaminase activity; IDA:UniProtKB.
 GO:0052656; F:L-isoleucine transaminase activity; IEA:EC.
 GO:0052654; F:L-leucine transaminase activity; IEA:EC.
 GO:0052655; F:L-valine transaminase activity; IEA:EC.
 GO:0030170; F:pyridoxal phosphate binding; IDA:UniProtKB.
 GO:0009081; P:branched-chain amino acid metabolic process; IDA:UniProtKB.
 GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0018272; P:protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; IDA:UniProtKB.
 GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR001544; Aminotrans_IV.
 IPR018300; Aminotrans_IV_CS.
 IPR005786; B_amino_transII. 

Pfam

 PF01063; Aminotran_4 

SMART

  

PROSITE

 PS00770; AA_TRANSFER_CLASS_4 

PRINTS