UniProt Accession

 TIM50_HUMAN; Q3ZCQ8; Q6QA00; Q96FJ5; Q96GY2; Q9H370 

Genbank Protein ID

 AY551341; BC009072; BC010736; BC050082; AF130109 

Genbank Nucleotide ID

 AAT01208.1; AAH09072.1; AAH10736.1; AAH50082.1; AAG35534.1 

Protein Name

 Mitochondrial import inner membrane translocase subunit TIM50 

Protein Synonyms/Alias

  

Gene Name

 TIMM50 

Gene Synonyms/Alias

 TIM50; PRO1512 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
47	IGSRGSTKAQGPQQQ	ubiquitination	[1]
64	SEGPSYAKKVALWLA	ubiquitination	[1]
232	YMDGHHVKDISCLNR	ubiquitination	[2]
312	DDPLAAFKQRQSRLE	ubiquitination	[1, 2, 3]
331	QRLAELSKSNKQNLF	ubiquitination	[1]
334	AELSKSNKQNLFLGS	ubiquitination	[1, 2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865] 

Functional Description

 Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. 

Sequence Annotation

 DOMAIN 143 286 FCP1 homology.  

Keyword

 Alternative splicing; Complete proteome; Direct protein sequencing; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleus; Protein transport; Reference proteome; RNA-binding; Transit peptide; Translocation; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 353 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005744; C:mitochondrial inner membrane presequence translocase complex; IPI:UniProtKB.
 GO:0016607; C:nuclear speck; IDA:UniProtKB.
 GO:0005134; F:interleukin-2 receptor binding; IDA:MGI.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:UniProtKB.
 GO:0004725; F:protein tyrosine phosphatase activity; IDA:UniProtKB.
 GO:0043021; F:ribonucleoprotein complex binding; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007006; P:mitochondrial membrane organization; IMP:UniProtKB.
 GO:0006626; P:protein targeting to mitochondrion; TAS:Reactome.
 GO:0001836; P:release of cytochrome c from mitochondria; IDA:MGI. 

Interpro

 IPR023214; HAD-like_dom.
 IPR004274; NIF.
 IPR027111; Tim50. 

Pfam

 PF03031; NIF 

SMART

 SM00577; CPDc 

PROSITE

 PS50969; FCP1 

PRINTS