CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009020
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase epsilon subunit 2 
Protein Synonyms/Alias
 DNA polymerase II subunit 2; DNA polymerase epsilon subunit B 
Gene Name
 POLE2 
Gene Synonyms/Alias
 DPE2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15SRALSAFKLRGLLLRubiquitination[1, 2, 3]
54KIINAVEKQPLSSNMubiquitination[3, 4, 5]
105VYNSERKKFLPLLMTubiquitination[3]
126LFGTPRDKAEMFRERubiquitination[3, 5]
162HPDESGSKFQLKTIEubiquitination[3, 4]
166SGSKFQLKTIETLLGubiquitination[1, 3, 4, 5]
191LGMITQLKEGKFFLEubiquitination[1, 3, 4]
194ITQLKEGKFFLEDPTubiquitination[3, 4, 5]
270GPSNTSVKTSAKLKQubiquitination[3, 4]
276VKTSAKLKQLEEENKubiquitination[4]
339KNQVQALKDSLKTLAubiquitination[3, 4]
422FREDLVNKMCRNCVRubiquitination[3]
517KVFYPSNKTVEDSKLubiquitination[3, 4, 5]
523NKTVEDSKLQGF***ubiquitination[3, 4, 5, 6]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Participates in DNA repair and in chromosomal DNA replication. 
Sequence Annotation
  
Keyword
 3D-structure; Alternative splicing; Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Nucleotidyltransferase; Nucleus; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 527 AA 
Protein Sequence
MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN 60
MIERSVVEAA VQECSQSVDE TIEHVFNIIG AFDIPRFVYN SERKKFLPLL MTNHPAPNLF 120
GTPRDKAEMF RERYTILHQR THRHELFTPP VIGSHPDESG SKFQLKTIET LLGSTTKIGD 180
AIVLGMITQL KEGKFFLEDP TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHVNAF 240
GFPPTEPSST TRAYYGNINF FGGPSNTSVK TSAKLKQLEE ENKDAMFVFL SDVWLDQVEV 300
LEKLRIMFAG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKTLADIIC EYPDIHQSSR 360
FVFVPGPEDP GFGSILPRPP LAESITNEFR QRVPFSVFTT NPCRIQYCTQ EITVFREDLV 420
NKMCRNCVRF PSSNLAIPNH FVKTILSQGH LTPLPLYVCP VYWAYDYALR VYPVPDLLVI 480
ADKYDPFTTT NTECLCINPG SFPRSGFSFK VFYPSNKTVE DSKLQGF 527 
Gene Ontology
 GO:0008622; C:epsilon DNA polymerase complex; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003887; F:DNA-directed DNA polymerase activity; TAS:ProtInc.
 GO:0006270; P:DNA replication initiation; TAS:Reactome.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0006297; P:nucleotide-excision repair, DNA gap filling; TAS:Reactome.
 GO:0000722; P:telomere maintenance via recombination; TAS:Reactome.
 GO:0032201; P:telomere maintenance via semi-conservative replication; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome. 
Interpro
 IPR007185; DNA_pol_alpha/epsilon_bsu.
 IPR016266; DNA_pol_e_bsu.
 IPR024639; DNA_pol_e_bsu_N. 
Pfam
 PF04042; DNA_pol_E_B
 PF12213; Dpoe2NT 
SMART
  
PROSITE
  
PRINTS