CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031086
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase UBR4 
Protein Synonyms/Alias
 cDNA FLJ53474, highly similar to Homo sapiens ubiquitin protein ligase E3 component n-recognin 4 (UBR4), mRNA 
Gene Name
 UBR4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
208LKDLILQKGITQNALphosphoglycerylation[1]
208LKDLILQKGITQNALubiquitination[2]
235LDADIWKKFLSRPALubiquitination[2]
325MAMAMRQKALGTLGMubiquitination[2]
337LGMTTNEKGQVVTKTubiquitination[2]
343EKGQVVTKTALLKQMubiquitination[2, 3]
383LGIYTFTKRVALEEMubiquitination[2]
Reference
 [1] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Ligase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 706 AA 
Protein Sequence
MRIVYRMRGL LGDATEEFIE SLDSTTDEEE DEEEVYKMAG VMAQCGGLEC MLNRLAGIRD 60
FKQGRHLLTV LLKLFSYCVK VKVNRQQLVK LEMNTLNVML GTLNLGNLLL TGDKDQLVML 120
LDQINSTFVR SNPSVLQGLL RIIPYLSFGE VEKMQILVER FKPYCNFDKY DEDHSGDDKV 180
FLDCFCKIAA GIKNNSNGHQ LKDLILQKGI TQNALDYMKK HIPSAKNLDA DIWKKFLSRP 240
ALPFILRLLR GLAIQHPGTQ VLIGTDSIPN LHKLEQVSSD EGIGTLAENL LEALREHPDV 300
NKKIDAARRE TRAEKKRMAM AMRQKALGTL GMTTNEKGQV VTKTALLKQM EELIEEPGLT 360
CCICREGYKF QPTKVLGIYT FTKRVALEEM ENKPRKQQGY STVSHFNIVH YDCHLAAVRL 420
ARGREEWESA ALQNANTKCN GLLPVWGPHV PESAFATCLA RHNTYLQECT GQREPTYQLN 480
IHDIKLLFLR FAMEQSFSAD TGGGGRESNI HLIPYIIHTV LYVLNTTRAT SREEKNLQGF 540
LEQPKEKWVE SAFEVDGPYY FTVLALHILP PEQWRATRVE ILRRLLVTSQ ARAVAPGGAT 600
RLTDKAVKDY SAYRSSLLFW ALVDLIYNMF KKVPTSNTEG GWSCSLAEYI RHNDMPIYEA 660
ADKALKTFQE EFMPVETFSE FLDVAGLLSE ITDPESFLKD LLNSVP 706 
Gene Ontology
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW. 
Interpro
 IPR025704; E3_Ub_ligase_UBR4. 
Pfam
 PF13764; E3_UbLigase_R4 
SMART
  
PROSITE
  
PRINTS