CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009969
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Na(+)/H(+) exchange regulatory cofactor NHE-RF1 
Protein Synonyms/Alias
 NHERF-1; Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na(+)/H(+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1 
Gene Name
 Slc9a3r1 
Gene Synonyms/Alias
 Nherf; Nherf1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
34HLHGEKGKVGQFIRLubiquitination[1]
50EPGSPAEKSGLLAGDubiquitination[1]
69VNGENVEKETHQQVVubiquitination[1]
101ETDERLKKLGVSIREubiquitination[1]
116ELLRPQEKSEQAEPPubiquitination[1]
139GDQNEAEKSHLRELRubiquitination[1]
169NLHSDKSKPGQFIRAubiquitination[1]
215GDVVSAIKGGGDEAKubiquitination[1]
228AKLLVVDKETDEFFKacetylation[2]
228AKLLVVDKETDEFFKubiquitination[1]
235KETDEFFKKCKVIPSacetylation[2]
261FSNGEIQKESSREALubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 
Functional Description
 Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling (By similarity). May participate in HTR4 targeting to microvilli. Involved in the regulation of phosphate reabsorption in the renal proximal tubules (By similarity). 
Sequence Annotation
 DOMAIN 14 94 PDZ 1.
 DOMAIN 149 229 PDZ 2.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 46 46 Phosphoserine (By similarity).
 MOD_RES 264 264 Phosphoserine (By similarity).
 MOD_RES 275 275 Phosphoserine.
 MOD_RES 283 283 Phosphoserine.
 MOD_RES 285 285 Phosphoserine.
 MOD_RES 286 286 Phosphoserine.
 MOD_RES 288 288 Phosphothreonine (By similarity).
 MOD_RES 289 289 Phosphoserine.
 MOD_RES 297 297 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Membrane; Phosphoprotein; Reference proteome; Repeat; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 355 AA 
Protein Sequence
MSADAAAGEP LPRLCCLEKG PNGYGFHLHG EKGKVGQFIR LVEPGSPAEK SGLLAGDRLV 60
EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDERLK KLGVSIREEL LRPQEKSEQA 120
EPPAAADTHE AGDQNEAEKS HLRELRPRLC TMKKGPNGYG FNLHSDKSKP GQFIRAVDPD 180
SPAEASGLRA QDRIVEVNGV CMEGKQHGDV VSAIKGGGDE AKLLVVDKET DEFFKKCKVI 240
PSQEHLDGPL PEPFSNGEIQ KESSREALVE PASESPRPAL ARSASSDTSE ELNSQDSPKR 300
QVSTEPSSTS SSSSDPILDL NISLAVAKER AHQKRSSKRA PQMDWSKKNE LFSNL 355 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IDA:MGI.
 GO:0031526; C:brush border membrane; IDA:MGI.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
 GO:0030175; C:filopodium; IEA:UniProtKB-SubCell.
 GO:0031528; C:microvillus membrane; ISS:UniProtKB.
 GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
 GO:0097225; C:sperm midpiece; IDA:UniProtKB.
 GO:0017081; F:chloride channel regulator activity; ISS:UniProtKB.
 GO:0050780; F:dopamine receptor binding; IDA:MGI.
 GO:0030036; P:actin cytoskeleton organization; IMP:MGI.
 GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; IMP:MGI.
 GO:0006915; P:apoptotic process; IEA:Compara.
 GO:0032782; P:bile acid secretion; IMP:UniProtKB.
 GO:0019933; P:cAMP-mediated signaling; IMP:MGI.
 GO:0030643; P:cellular phosphate ion homeostasis; IMP:MGI.
 GO:0034613; P:cellular protein localization; IMP:MGI.
 GO:0034635; P:glutathione transport; IMP:UniProtKB.
 GO:0030033; P:microvillus assembly; IEA:Compara.
 GO:0030336; P:negative regulation of cell migration; IGI:MGI.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IEA:Compara.
 GO:0014067; P:negative regulation of phosphatidylinositol 3-kinase cascade; IMP:UniProtKB.
 GO:0010642; P:negative regulation of platelet-derived growth factor receptor signaling pathway; IMP:UniProtKB.
 GO:0051898; P:negative regulation of protein kinase B signaling cascade; IMP:UniProtKB.
 GO:0010766; P:negative regulation of sodium ion transport; IMP:MGI.
 GO:0032416; P:negative regulation of sodium:hydrogen antiporter activity; IMP:MGI.
 GO:0060158; P:phospholipase C-activating dopamine receptor signaling pathway; IMP:MGI.
 GO:0044062; P:regulation of excretion; IMP:MGI.
 GO:0045859; P:regulation of protein kinase activity; IMP:UniProtKB.
 GO:0097291; P:renal phosphate ion absorption; ISS:UniProtKB.
 GO:0003096; P:renal sodium ion transport; IMP:MGI.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR015098; EBP50_C-term.
 IPR017300; NaH_exchngr_reg_CF_NHE-RF.
 IPR001478; PDZ. 
Pfam
 PF09007; EBP50_C-term
 PF00595; PDZ 
SMART
 SM00228; PDZ 
PROSITE
 PS50106; PDZ 
PRINTS