CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006411
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 4 
Protein Synonyms/Alias
 Deubiquitinating enzyme 4; Ubiquitin thioesterase 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein 
Gene Name
 Usp4 
Gene Synonyms/Alias
 Unp 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
167DTIATIEKEMRKLFNacetylation[1]
186RETRLWNKYMSNTYEubiquitination[2]
232PRQSLQSKSSTAPSRubiquitination[2]
376HVAPRMFKTQVGRFAubiquitination[2]
588PVYFREKKSRPSSASubiquitination[2]
695DHSESAQKVKGQPRHubiquitination[2]
730ATDGKLLKLNSRSTLubiquitination[2]
769LSMSQPQKKKKAAVAubiquitination[2]
810KHQQATKKFDLWSLPubiquitination[2]
836YNRYWRDKLDTVVEFubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER (By similarity). 
Sequence Annotation
 DOMAIN 11 122 DUSP.
 DOMAIN 142 226 Ubiquitin-like 1.
 DOMAIN 483 571 Ubiquitin-like 2.
 REGION 405 407 Necessary for interaction with RBL2.
 REGION 459 463 Necessary for interaction with RB1 and
 MOTIF 133 141 Nuclear export signal.
 MOTIF 766 771 Nuclear localization signal.
 ACT_SITE 311 311 Nucleophile (By similarity).
 ACT_SITE 880 880 Proton acceptor (By similarity).
 METAL 461 461 Zinc (By similarity).
 METAL 464 464 Zinc (By similarity).
 METAL 798 798 Zinc (By similarity).
 METAL 801 801 Zinc (By similarity).
 MOD_RES 680 680 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Nucleus; Phosphoprotein; Protease; Proto-oncogene; Reference proteome; Repeat; Thiol protease; Ubl conjugation; Ubl conjugation pathway; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 962 AA 
Protein Sequence
MAEGRGSRER PDVETQKTEL GALMGTTLQR GAQWYLIDSR WFKQWKKYVG FDSWDMYNVG 60
EHNLFPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPAEA WNKLLNWYGC VEGQQPIVRK 120
VVEHGLFVKH CKVEVYLLEL KLCENSDPTN VLSCHFSKAD TIATIEKEMR KLFNIPAERE 180
TRLWNKYMSN TYEQLSKLDN TIQDAGLYQG QVLVIEPQNE DGTWPRQSLQ SKSSTAPSRN 240
FTTSSKPSAS PYCSVSASLI ANGDSTNSSG MHSSGVSRGG SGFSASYNCQ EPPSPHIQPG 300
LCGLGNLGNT CFMNSALQCL SNTAPLTEYF LKDEYEAEIN RDNPLGMKGE IAEAYAELIK 360
QMWSGRDTHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF ILDGLHEDLN RVKKKPYLEP 420
KDANGRPDAV VAKEAWENHR LRNDSVIVDT FHGLFKSTLV CPECAKVSVT FDPFCYLTLP 480
LPLKKDRIME VFLVPADPQC RPIQYRVTVP LMGAISDLCE ALSKLSGIAA ENMVVTDVYN 540
HRFHKIFQMD EGLSHITPRD DIFVYEVCNT SMDGSECITL PVYFREKKSR PSSASSGAVL 600
YGQPLLVSVP KHKLTLESLY QAVCDRISRY IKQPLPDEFL SSPLEPGACN GSRSSYEGDE 660
EEEMDHQEEG KEQLSEVEGS GEDDQGDDHS ESAQKVKGQP RHKRLFTFSL VNSCGTADIN 720
SLATDGKLLK LNSRSTLAID WDSETRSLYF DEQESEACEK HLSMSQPQKK KKAAVALREC 780
IELFTTMETL GEHDPWYCPT CKKHQQATKK FDLWSLPKIL VVHLKRFSYN RYWRDKLDTV 840
VEFPVRALNM SEFVCDRSAR PYVYDLIAVS NHYGAMGVGH YTAYAKNRLN GKWYYFDDSS 900
VSLASEDQIV TKAAYVLFYQ RRDDECSSTS SLGSFPGSDG GVKLSSSHQG MGDEEAYNMD 960
TN 962 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
 GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
 GO:0016579; P:protein deubiquitination; ISS:UniProtKB.
 GO:0034394; P:protein localization to cell surface; ISS:UniProtKB.
 GO:0031647; P:regulation of protein stability; ISS:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR006615; Pept_C19_DUSP.
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF06337; DUSP
 PF00443; UCH 
SMART
 SM00695; DUSP 
PROSITE
 PS51283; DUSP
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS