CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010378
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Protein GrpE 
Protein Synonyms/Alias
 HSP-70 cofactor 
Gene Name
 grpE 
Gene Synonyms/Alias
 MPN_120; MP034 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
21SIRTLLVKHNKAKVTacetylation[1]
32AKVTQIEKELLQAVAacetylation[1]
43QAVAELEKKFKQQVQacetylation[1]
64QKIPNLQKVNEEFRLacetylation[1]
96AELTIKSKEELENAKacetylation[1]
197KVSKSGYKLHDKVIRacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819
Functional Description
 Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP- dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding (By similarity). 
Sequence Annotation
  
Keyword
 Chaperone; Complete proteome; Cytoplasm; Reference proteome; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 217 AA 
Protein Sequence
MSENSLTITE ILSSIRTLLV KHNKAKVTQI EKELLQAVAE LEKKFKQQVQ NFNELQQKIP 60
NLQKVNEEFR LKVEKIQEEA QKKIQEKVAE LTIKSKEELE NAKKYVIEKS IDQPLIIIDQ 120
FEIALSYAQK DPQVKNYTTG FNMVLDAFSR WLEGFGVTKI AIEPGAQFDE KVMAALEVVP 180
SDQPANTVVK VSKSGYKLHD KVIRFASVVV SQGNKTE 217 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000774; F:adenyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 
Interpro
 IPR000740; GrpE.
 IPR013805; GrpE_coiled_coil.
 IPR009012; GrpE_head. 
Pfam
 PF01025; GrpE 
SMART
  
PROSITE
 PS01071; GRPE 
PRINTS
 PR00773; GRPEPROTEIN.