CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010147
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Glutamyl-tRNA(Gln) amidotransferase subunit A 
Protein Synonyms/Alias
 Glu-ADT subunit A 
Gene Name
 gatA 
Gene Synonyms/Alias
 MPN_237; MP594 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
7*MKSQILKLQQTLTKacetylation[1]
231KADFSAQKSPDAGKFacetylation[1]
237QKSPDAGKFTRSLKEacetylation[1]
368TDKNNFTKYFEKAQKacetylation[1]
372NFTKYFEKAQKIRRVacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity). 
Sequence Annotation
 ACT_SITE 68 68 Charge relay system (By similarity).
 ACT_SITE 143 143 Charge relay system (By similarity).
 ACT_SITE 167 167 Acyl-ester intermediate (By similarity).  
Keyword
 ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 478 AA 
Protein Sequence
MKSQILKLQQ TLTKKPASIN PLLQQIDGAI NEHWSSNFLL KNTVEWAQAQ APKNRSKSPL 60
NNIPFVLKDN IATKGIVTTG GSRFLEDYIP PFSATVFELL NNSGALLVGK ANLDEFGLGG 120
TGLHSGFGFV HHPWNETLIP GGSSSGSAYA VARGIVPFSI GTDTGDSVRR PASICNIVGF 180
KPTYGLISRN GVYPYAPSLD HVGIFARYVY DVALVSDEII KHDKADFSAQ KSPDAGKFTR 240
SLKESFNKQI KIGYLKPLEE WFDIELSKKW NSLKERITLE GCELIPFHFP LELLEVIDPV 300
YKLISYSEAV SCYSNLTGIV FGQKLFEPNQ ASDFSKTITA NRDRFFGEQL KRRFIIGAFG 360
TDKNNFTKYF EKAQKIRRVM VDAYLNLFKE ADFIVSPSAS GFTKTIAAVQ KGESFTNLVD 420
DFLQLANFAG NPSITIPWLV KQKDQTIGLS VNANCFHDKQ LLQVAAWLEE LFQIEHDD 478 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0050567; F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; IEA:HAMAP.
 GO:0006412; P:translation; IEA:HAMAP. 
Interpro
 IPR000120; Amidase.
 IPR020556; Amidase_CS.
 IPR023631; Amidase_dom.
 IPR004412; GatA. 
Pfam
 PF01425; Amidase 
SMART
  
PROSITE
 PS00571; AMIDASES 
PRINTS