CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036875
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nesprin-1 
Protein Synonyms/Alias
  
Gene Name
 SYNE1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
3708NVATKIDKVDTVMMGubiquitination[1]
3716VDTVMMGKKLKTLEVubiquitination[1]
4198FQLEDLIKRKIQVSVmethylation[2]
5097IKEVEQSKATSQELSubiquitination[1]
5109ELSRQIQKLAKDLTTubiquitination[1]
5548LEPSATAKLGDLQRSubiquitination[3]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 8409 AA 
Protein Sequence
MATSRGASRC PRDIANVMQR LQDEQEIVQK RTFTKWINSH LAKRKPPMVV DDLFEDMKDG 60
VKLLALLEVL SGQKLPCEQG RRMKRIHAVA NIGTALKFLE GRKIKLVNIN STDIADGRPS 120
IVLGLMWTII LYFQIEELTS NLPQLQSLSS SASSVDSIVS SETPSPPSKR KVTTKIQGNA 180
KKALLKWVQY TAGKQTGIEV KDFGKSWRSG VAFHSVIHAI RPELVDLETV KGRSNRENLE 240
DAFTIAETEL GIPRLLDPED VDVDKPDEKS IMTYVAQFLK HYPDIHNAST DGQEDDEILP 300
GFPSFANSVQ NFKREDRVIF KEMKVWIEQF ERDLTRAQMV ESNLQDKYQS FKHFRVQYEM 360
KRKQIEHLIQ PLHRDGKLSL DQALVKQSWD RVTSRLFDWH IQLDKSLPAP LGTIGAWLYR 420
AEVALREEIT VQQVHEETAN TIQRKLEQHK RKLRNVIDLL QNTDAHKRAF HEIYRTRSVN 480
GIPVPPDQLE DMAERFHFVS STSELHLMKM EFLELKYRLL SLLVLAESKL KSWIIKYGRR 540
ESVEQLLQNY VSFIENSKFF EQYEVTYQIL KQTAEMYVKA DGSVEEAENV MKFMNETTAQ 600
WRNLSVEVRS VRSMLEEVIS NWDRYGNTVA SLQAWLEDAE KMLNQSENAK KDFFRNLPHW 660
IQQHTAMNDA GNFLIETCDE MVSRDLKQQL LLLNGRWREL FMEVKQYAQA DEMDRMKKEY 720
TDCVVTLSAF ATEAHKKLSE PLEVSFMNVK LLIQDLEDIE QRVPVMDAQY KIITKTAHLI 780
TKESPQEEGK EMFATMSKLK EQLTKVKECY SPLLYESQQL LIPLEELEKQ MTSFYDSLGK 840
INEIITVLER EAQSSALFKQ KHQLRILKCK WCNSSQKMTL CLVLGSMISM LSTAKITIAG 900
YKSEVEVSEL LACQENCKKT LTLIEKGSQS VQKFVTLSNV LKHFDQTRLQ TDCRYSCCFS 960
VMRKQHHIMI VFHIKSMVKK TGDWKKHVET NSRLMKKFEE SRAELEKVLR IAQEGLEEKG 1020
DPEELLRRHT EFFSQLDQRV LNAFLKACDE LTDILPEQEQ QGLQEAVRKL HKQWKDLQGE 1080
APYHLLHLKI DVEKNRFLAS VEECRTELDR ETKLMPQEGS EKIIKEHRVF FSDKGPHHLC 1140
EKRLQLIEEL CVKLPVRDPV RDTPGTCHVT LKELRAAIDS TYRKLMEDPD KWKDYTSRFS 1200
EFSSWISTNE TQLKGIKGEA IDTANHGEVK RAVEEIRNGV TKRGETLSWL KSRLKVLTEV 1260
SSENEAQKQG DELAKLSSSF KALVTLLSEV EKMLSNFGDC VQYKEIVKNS LEELISGSKE 1320
VQEQAEKILD TENLFEAQQL LLHHQQKTKR ISAKKRDVQQ QIAQAQQGEG GLPDRGHEEL 1380
RKLESTLDGL ERSRERQERR IQVTLRKWER FETNKETVVR YLFQTGSSHE RFLSFSSLES 1440
LSSELEQTKE FSKRTESIAV QAENLVKEAS EIPLGPQNKQ LLQQQAKSIK EQVKKLEDTL 1500
EEEYVIDNKT MEMVKTKWDH FGSNFETLSV WITEKEKELN ALETSSSAMD MQISQIKVTI 1560
QEIESKLSSI VGLEEEAQSF AQFVTTGESA RIKAKLTQIR RYGEELREHA QCLEGTILGH 1620
LSQQQKFEEN LRKIQQSVSE FEDKLAVPIK ICSSATETYK VLQEHMDLCQ ALESLSSAIT 1680
AFSASARKVV NRDSCVQEAA ALQQQYEDIL RRAKERQTAL ENLLAHWQRL EKELSSFLTW 1740
LERGEAKASS PEMDISADRV KVEASKDDVK MMKLHLEQLD ERWRDLPQII NKRINFLQSV 1800
VAEHQQFDEL LLSFSVWIKL FLSELQTTSE ISIMDHQVAL TRHKDHAAEV ESKKGELQSL 1860
QGHLAKLGSL GRAEDLHLLQ GKAEDCFQLF EEASQVVERR QLALSHLAEF LQSHASLSGI 1920
LRQLRQTVEA TNSMNKNESD LIEKDLNDAL QNAKALESAA VSLDGILSKA QYHLKIGSSE 1980
QRTSCRATAD QLCGEVERIQ NLLGTKQSEA DALAVLKKAF QDQKEELLKS IEDIEERTDK 2040
ERLKEPTRQA LQQRLRVFNQ LEDELNSHEH ELCWLKDKAK QIAQKDVAFA PEVDREINRL 2100
EVTWDDTKRL IHENQGQCCG LIDLMREYQN LKSAVSKVLE NASSVIVTRT TIKDQEDLKW 2160
AFSKHETAKN KMNYKQKDLD NFTSKGKHLL SELKKIHSSD FSLVKTDMES TVDKWLDVSE 2220
KLEENMDRLR VSLSIWDDVL STRDEIEGWS NNCVPQMAEN ISNLDNHLRA EELLKEFESE 2280
VKNKALRLEE LHSKVNDLKE LTKNLETPPD LQFIEADLMQ KLEHAKEITE VAKGTLKDFT 2340
AQSTQVEKFI NDITTWFTKV EESLMNCAQN ETCEALKKVK DIQKELQSQQ SNISSTQENL 2400
NSLCRKYHSA ELESLGRAMT GLIKKHEAVS QLCSKTQASL QESLEKHFSE ESMQEFQEWF 2460
LGAKAAAKES SDRTGDSKVL EAKLHDLQQN ILDSVSDGQS KLDAVTQEGQ TLYAHLSKQI 2520
VSSIQEQITK ANEEFQAFLK QCLKDKQALQ DCASELGSFE DQHRKLNLWI HEMEERFNTE 2580
NLGESKQHIP EKKNEVHKVE MFLGELLAAR ESLDKLSQRG QLLSEEGHGA GQEGRLCSQL 2640
LTSHQNLLRM TKEKLRSCQV ALQEHEALEE ALQSMWFWVK AIQDRLACAE STLGSKDTLE 2700
KRLSQIQDIL LMKGEGEVKL NMAIGKGEQA LRSSNKEGQR VIQTQLETLK EVWADIMSSS 2760
VHAQSTLESV ISQWNDYVER KNQLEQWMES VDQKIEHPLQ PQPGLKEKFV LLDHLQSILS 2820
EAEDHTRALH RLIAKSRELY EKTEDESFKD TAQEELKTQF NDIMTVAKEK MRKVEEIVKD 2880
HLMYLDAVHE FTDWLHSAKE ELHRWSDMSG DSSATQKKLS KIKELIDSRE IGASRLSRVE 2940
SLAPEVKQNT TASGCELMHT EMQALRADWK QWEDSVFQTQ SCLENLVSQM ALSEQEFSGQ 3000
VAQLEQALEQ FSALLKTWAQ QLTLLEGKNT DEEIVECWHK GQEILDALQK AEPRTEDLKS 3060
QLNELCRFSR DLSTYSGKVS GLIKEYNCLC LQASKGCQNK EQILQQRFRK AFRDFQQWLV 3120
NAKITTAKCF DIPQNISEVS TSLQKIQEFL SESENGQHKL NMMLSKGELL STLLTKEKAK 3180
GIQAKVTAAK EDWKNFHSNL HQKESALENL KIQMKDFEVS AEPIQDWLSK TEKMVHESSN 3240
RLYDLPAKRR EQQKLQSVLE EIHCYEPQLN RLKEKAQQLW EGQAASKSFR HRVSQLSSQY 3300
LALSNLTKEK VSRLDRIVAE HNQFSLGIKE LQDWMTDAIH MLDSYCHPTS DKSVLDSRTL 3360
KLEALLSVKQ EKEIQMKMIV TRGESVLQNT SPEGIPTIQQ QLQSVKDMWA SLLSAGIRCK 3420
RVRLPCAYIA LKLRVHQHAE LRDKTTMLGK AKEAVTKSEK LVRLHQEYQR DLKAFEVWLG 3480
QEQEKLDQYS VLEGDAHTHE TTLRDLQELQ VHCAEGQALL NSVLHTREDV IPSGIPQAED 3540
RALESLRQDW QAYQHRLSET RTQFNNVVNK LRLMEQKFQQ VDEWLKTAEE KVSPRTRRQS 3600
NRATKEIQLH QMKKWHEEVT AYRDEVEEVG ARAQEILDES HVNSRMGCQA TQLTSRYQAL 3660
LLQVLEQIKF LEEEIQSLEE SESSLSSYSD WYGSTHKNFK NVATKIDKVD TVMMGKKLKT 3720
LEVLLKDMEK GHSLLKSARE KGERAVKYLE EGEAERLRKE IHDHMEQLKE LTSTVRKEHM 3780
TLEKSLQQTV LSHEPSVKSV REKGEALLEL VQDVTLKDKI DQLQSDYQDL CSIGKEHVFS 3840
LEAKVKDHED YNSELQEVEK WLLQMSGRLV APDLLETSSL ETITQQLAHH KMYQSLEHEL 3900
QKHVSRQDTL QQCQAWLSAV QPDLEPSPQP PLSRAEAIKQ VKHFRALQEQ ARTYLDLLCS 3960
MCDLSNASVK TTAKDIQQTE QTIEQKLVQA QNLTQGWEEI KHLKSELWIY LQDADQQLQN 4020
MKRRHSELEL NIAQNMVSQV KDFVKKLQSK QASVNTIIEK VNKLTKKEES PEHKEINHLN 4080
DQWLDLCRQS NNLCLQREED LQRTRDYHDC MNVVEVFLEK FTTEWDNLAR SDAESTAVHL 4140
EALKKLALAL QERKYAIEDL KDQKQKMIEH LNLDDKELVK EQTSHLEQRW FQLEDLIKRK 4200
IQVSVTNLEE LNVVQSRFQE LMEWAEEQQP NIAEALKQSP PPDMAQNLLM DHLAICSELE 4260
AKQMLLKSLI KDADRVMADL GLNERQVIQK ALSDAQSHVN CLSDLVGQRR KKHFKEDFDK 4320
ACHWLKQADI VTFPEINLMN ESSELHTQLA KYQNILEQSP EYENLLLTLQ RTGQTILPSL 4380
NEVDHSYLSE KLNALPRQFN VIVALAKDKF YKVQEAILAR KEYASLIELT TQSLSELEAQ 4440
FLRMSKVPTD LAVEEALSLQ DGCRAILDEV AGLGEAVDEL NQKKEGFRST GQPWQPDKML 4500
HLVTLYHRLK RQTEQRVSLL EDTTSAYQEH EKMCQQLERQ LKSVKEEQSK VNEETLPAEE 4560
KLKMYHSLAG SLQDSGIVLK RVTIHLEDLA PHLDPLAYEK ARHQIQSWQG ELKLLTSAIG 4620
ETVTECESRM VQSIDFQTEM SRSLDWLRRV KAELSGPVYL DLNLQDIQEE IRKIQIHQEE 4680
VQSSLRIMNA LSHKEKEKFT KAKELISADL EHSLAELSEL DGDIQEALRT RQATLTEIYS 4740
QCQRYYQVFQ AANDWLEDAQ ELLQLAGNGL DVESAEENLK SHMEFFSTED QFHSNLEELH 4800
SLVATLDPLI KPTGKEDLEQ KVASLELRSQ RMSRDSGAQA LVSVVNSFHE KIVALEEKAS 4860
QLEKTGNDAS KATLSRSMTT VWQRWTRLRA VAQDQEKILE DAVDEWTGFN NKVKKATEMI 4920
DQLQDKLPGS SAEKASKAEL LTLLEYHDTF VLELEQQQSA LGMLRQQTLS MLQDGAAPTP 4980
GEEPPLMQEI TAMQDRCLKM VETQINSVKC WVQETKEYLG NPTIEIDAQL EELQILLTEA 5040
TNHRQNIEKM AEEQKEKYLG LYTILPSELS LQLAEVALDL KIRDQIQDKI KEVEQSKATS 5100
QELSRQIQKL AKDLTTILTK LKAKTDNVVQ AKTDQKAASH LEEYNEMLEL ILKWIEKAKV 5160
LAHGTIAWNS ASQLREQYIL HQTLLEESKE IDSELEAMTE KLQYLTSVYC TEKMSQQVAE 5220
LGRETEELRQ MIKIRLQNLQ DAAKDMKKFE AELKKLQAAL EQAQATLTSP EVGRLSLKEQ 5280
LSHRQHLLSE MESLKPKVQA VQLCQSALRI PEDVVASLPL CHAALRLQEE ASRLQHTAIQ 5340
QCNIMQEAVV QYEQYEQEMK HLQQLIEGAH REIEDKPVAT SNIQELQAQI SRHEELAQKI 5400
KGYQEQIASL NSKCKMLTMK AKHATMLLTV TEVEGLAEGT EDLDGELLPT PSAHPSVVMM 5460
TAGRCHTLLS PVTEESGEEG TNSEISSPPA CRSPSPVANT DASVNQDIAY YQALSAERLQ 5520
TDAAKIHPST SASQEFYEPG LEPSATAKLG DLQRSWETLK NVISEKQRTL YEALERQQKY 5580
QDSLQSISTK MEAIELKLSE SPEPGRSPES QMAEHQALMD EILMLQDEIN ELQSSLAEEL 5640
VSESCEADPA EQLALQSTLT VLAERMSTIR MKASGKRQLL EEKLNDQLEE QRQEQALQRY 5700
RCEADELDSW LLSTKATLDT ALSPPKEPMD MEAQLMDCQN MLVEIEQKVV ALSELSVHNE 5760
NLLLEGKAHT KDEAEQLAGK LRRLKGSLLE LQRALHDKQL NMQGTAQEKE ESDVDLTATQ 5820
SPGVQEWLAQ ARTTWTQQRQ SSLQQQKELE QELAEQKSLL RSVASRGEEI LIQHSAAETS 5880
GDAGEKPDVL SQELGMEGEK SSAEDQMRMK WESLHQEFST KQKLLQNVLE QEQEQVLYSR 5940
PNRLLSGVPL YKGDVPTQDK SAVTSLLDGL NQAFEEVSSQ SGGAKRQSIH LEQKLYDGVS 6000
ATSTWLDDVE ERLFVATALL PEETETCLFN QEILAKDIKE MSEEMDKNKN LFSQAFPENG 6060
DNRDVIEDTL GCLLGRLSLL DSVVNQRCHQ MKERLQQILN FQNDLKVLFT SLADNKYIIL 6120
QKLANVFEQP VAEQIEAIQQ AEDGLKEFDA GIIELKRRGD KLQVEQPSMQ ELSKLQDMYD 6180
ELMMIIGSRR SGLNQNLTLK SQYERALQDL ADLLETGQEK MAGDQKIIVS SKEEIQQLLD 6240
KHKEYFQGLE SHMILTETLF RKIISFAVQK ETQFHTELMA QASAVLKRAH KRGVELEYIL 6300
ETWSHLDEDQ QELSRQLEVV ESSIPSVGLV EENEDRLIDR ITLYQHLKSS LNEYQPKLYQ 6360
VLDDGKRLLI SISCSDLESQ LNQLGECWLS NTNKMSKELH RLETILKHWT RYQSESADLI 6420
HWLQSAKDRL EFWTQQSVTV PQELEMVRDH LNAFLEFSKE VDAQSSLKSS VLSTGNQLLR 6480
LKKVDTATLR SELSRIDSQW TDLLTNIPAV QEKLHQLQMD KLPSRHAISE VMSWISLMEN 6540
VIQKDEDNIK NSIGYKAIHE YLQKYKGFKI DINCKQLTVD FVNQSVLQIS SQDVESKRSD 6600
KTDFAEQLGA MNKSWQILQG LVTEKIQLLE GLLESWSEYE NNVQCLKTWF ETQEKRLKQQ 6660
HRIGDQASVQ NALKDCQDLE DLIKAKEKEV EKIEQNGLAL IQNKKEDVSS IVMSTLRELG 6720
QTWANLDHMV GQLKILLKSV LDQWSSHKVA FDKINSYLME ARYSLSRFRL LTGSLEAVQV 6780
QVDNLQNLQD DLEKQERSLQ KFGSITNQLL KECHPPVTET LTNTLKEVNM RWNNLLEEIA 6840
EQLQSSKALL QLWQRYKDYS KQCASTVQQQ EDRTNELLKA ATNKDIADDE VATWIQDCND 6900
LLKGLGTVKD SLFFLHELGE QLKQQVDASA ASAIQSDQLS LSQHLCALEQ ALCKQQTSLQ 6960
AGVLDYETFA KSLEALEAWI VEAEEILQGQ DPSHSSDLST IQERMEELKG QMLKFSSMAP 7020
DLDRLNELGY RLPLNDKEIK RMQNLNRHWS LISSQTTERF SKLQSFLLQH QTFLEKCETW 7080
MEFLVQTEQK LAVEISGNYQ HLLEQQRAHE LFQAEMFSRQ QILHSIIIDG QRLLEQGQVD 7140
DRDEFNLKLT LLSNQWQGVI RRAQQRRGII DSQIRQWQRY REMAEKLRKW LVEVSYLPMS 7200
GLGSVPIPLQ QARTLFDEVQ FKEKVFLRQQ GSYILTVEAG KQLLLSADSG AEAALQAELA 7260
EIQEKWKSAS MRLEEQKKKL AFLLKDWEKC EKGIADSLEK LRTFKKKLSQ SLPDHHEELH 7320
AEQMRCKELE NAVGSWTDDL TQLSLLKDTL SAYISADDIS ILNERVELLQ RQWEELCHQL 7380
SLRRQQIGER LNEWAVFSEK NKELCEWLTQ MESKVSQNGD ILIEEMIEKL KKDYQEEIAI 7440
AQENKIQLQQ MGERLAKASH ESKASEIEYK LGKVNDRWQH LLDLIAARVK KLKETLVAVQ 7500
QLDKNMSSLR TWLAHIESEL AKPIVYDSCN SEEIQRKLNE QQELQRDIEK HSTGVASVLN 7560
LCEVLLHDCD ACATDAECDS IQQATRNLDR RWRNICAMSM ERRLKIEETW RLWQKFLDDY 7620
SRFEDWLKSS ERTAAFPSSS GVIYTVAKEE LKKFEAFQRQ VHECLTQLEL INKQYRRLAR 7680
ENRTDSACSL KQMVHEGNQR WDNLQKRVTS ILRRLKHFIG QREEFETARD SILVWLTEMD 7740
LQLTNIEHFS ECDVQAKIKQ LKAFQQEISL NHNKIEQIIA QGEQLIEKSE PLDAAIIEEE 7800
LDELRRYCQE VFGRVERYHK KLIRLPLPDD EHDLSDRELE LEDSAALSDL HWHDRSADSL 7860
LSPQPSSNLS LSLAQPLRSE RSGRDTPASV DSIPLEWDHD YDLSRDLESA MSRALPSEDE 7920
EGQDDKDFYL RGAVGLSGDH SALESQIRQL GKALDDSRFQ IQQTENIIRS KTPTGPELDT 7980
SYKGYMKLLG ECSSSIDSVK RLEHKLKEEE ESLPGFVNLH STETQTAGVI DRWELLQAQA 8040
LSKELRMKQN LQKWQQFNSD LNSIWAWLGD TEEELEQLQR LELSTDIQTI ELQIKKLKEL 8100
QKAVDHRKAI ILSINLCSPE FTQADSKESR DLQDRLSQMN GRWDRVCSLL EEWRGLLQDA 8160
LMQCQGFHEM SHGLLLMLEN IDRRKNEIVP IDSNLDAEIL QDHHKQLMQI KHELLESQLR 8220
VASLQDMSCQ LLVNAEGTDC LEAKEKVHVI GNRLKLLLKE VSRHIKELEK LLDVSSSQQD 8280
LSSWSSADEL DTSGSVSPTS GRSTPNRQKT PRGKCSLSQP GPSVSSPHSR STKGGSDSSL 8340
SEPGPGRSGR GFLFRVLRAA LPLQLLLLLL IGLACLVPMS EEDYSCALSN NFARSFHPML 8400
RYTNGPPPL 8409 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0031965; C:nuclear membrane; IDA:HPA. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR012315; KASH.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF10541; KASH
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS51049; KASH 
PRINTS